YUH1/YJR099W Summary Help

Standard Name YUH1 1, 2
Systematic Name YJR099W
Feature Type ORF, Verified
Description Ubiquitin C-terminal hydrolase; cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p (3, 4, 5)
Name Description Yeast Ubiquitin Hydrolase
Chromosomal Location
ChrX:615576 to 616286 | ORF Map | GBrowse
Gbrowse
Genetic position: 57 cM
Gene Ontology Annotations All YUH1 GO evidence and references
  View Computational GO annotations for YUH1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
39 total interaction(s) for 32 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 4
  • Co-crystal Structure: 1
  • Reconstituted Complex: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 29
  • Positive Genetic: 1
  • Synthetic Growth Defect: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 236
Molecular Weight (Da) 26,385
Isoelectric Point (pI) 4.28
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:615576 to 616286 | ORF Map | GBrowse
SGD ORF map
Genetic position: 57 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..711 615576..616286 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003860
References cited on this page View Complete Literature Guide for YUH1
1) Game, J.C., et al.  (1992) Personal Communication, Mortimer Map Edition 11
2) Miller H, et al.  (1989) Cloning and Expression of a Yeast Ubiquitin-Protein Cleaving Activity in Escherichia Coli Biotechnology 7():698-704
3) Sakamoto T, et al.  (1999) An NMR analysis of ubiquitin recognition by yeast ubiquitin hydrolase: evidence for novel substrate recognition by a cysteine protease. Biochemistry 38(36):11634-42
4) Linghu B, et al.  (2002) Rub1p processing by Yuh1p is required for wild-type levels of Rub1p conjugation to Cdc53p. Eukaryot Cell 1(3):491-4
5) Larsen CN, et al.  (1998) Substrate specificity of deubiquitinating enzymes: ubiquitin C-terminal hydrolases. Biochemistry 37(10):3358-68