YRF1-1/YDR545W Summary Help

Standard Name YRF1-1
Systematic Name YDR545W
Alias YRF1
Feature Type ORF, Verified
Description Helicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p (1, 2 and see Summary Paragraph)
Chromosomal Location
ChrIV:1526321 to 1531711 | ORF Map | GBrowse
Gene Ontology Annotations All YRF1-1 GO evidence and references
  View Computational GO annotations for YRF1-1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
12 total interaction(s) for 9 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 2
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 1

Expression Summary
Length (a.a.) 1,796
Molecular Weight (Da) 203,779
Isoelectric Point (pI) 7.49
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:1526321 to 1531711 | ORF Map | GBrowse
This feature contains embedded feature(s): YDR545C-A
This feature is contained within: TEL04R
This feature is contained within: TEL04R-YP
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..5391 1526321..1531711 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002953

The YRF1 genes are found in telomeric Y' elements and encode a DNA helicase known as Y'-Help1 (Y'-HELicase Protein 1), which is induced in strains deficient for telomerase activity (1). Native Y' elements do not appear to be expressed in wild-type cells, probably due to the telomeric silencing effect (3). Thus, Y'-Help1 expression from shortened telomeres, in which silencing is no longer effective, seems to indicate the presence of a system for the compensation of telomeric loss by telomerase-independent mechanisms (1). It is proposed that the induction of Y'-Help1 promotes homologous DNA recombination among Y' elements, thereby facilitating their amplification, resulting in the lengthening of telomeric ends and prevention of chromosome loss and cell death. This amplification of Y' elements will further increase the production of Y'-Help1, establishing a positive feedback circuit for telomeric rescue in cells with shortened telomeres (1).

The N-terminal region of Y'-Help1 is composed of motifs conserved in the DEAD/DEAH (or DEXH) subfamily of helicases, and the central region of the protein contains several tandem repeats of the serine-threonine-rich 12-amino acid sequence SXTXATTTXSXX, the number of which varies between the different Y' elements (1). Y'-Help1 also shares regions of homology with the RNA helicase eukaryotic translation initiation factor 4A (Tif1p and Tif2p in S. cerevisiae), as well as the homologous DNA recombination protein RecG of the hyperthermophilic bacterium Aquifex (1).

Last updated: 2006-06-27 Contact SGD

References cited on this page View Complete Literature Guide for YRF1-1
1) Yamada M, et al.  (1998) Y'-Help1, a DNA helicase encoded by the yeast subtelomeric Y' element, is induced in survivors defective for telomerase. J Biol Chem 273(50):33360-6
2) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
3) Gottschling DE, et al.  (1990) Position effect at S. cerevisiae telomeres: reversible repression of Pol II transcription. Cell 63(4):751-62