YPT7/YML001W Summary Help

Standard Name YPT7 1
Systematic Name YML001W
Alias AST4 , VAM4 2
Feature Type ORF, Verified
Description Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7 (1, 3, 4, 5, 6 and see Summary Paragraph)
Name Description Yeast Protein Two 7
Chromosomal Location
ChrXIII:267174 to 267800 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All YPT7 GO evidence and references
  View Computational GO annotations for YPT7
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
Resources
276 total interaction(s) for 200 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 23
  • Biochemical Activity: 4
  • Co-fractionation: 4
  • Co-localization: 1
  • Co-purification: 4
  • PCA: 6
  • Protein-RNA: 1
  • Reconstituted Complex: 10
  • Two-hybrid: 21

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 6
  • Negative Genetic: 128
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 4
  • Positive Genetic: 28
  • Synthetic Growth Defect: 15
  • Synthetic Lethality: 4
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 208
Molecular Weight (Da) 23,043
Isoelectric Point (pI) 4.62
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:267174 to 267800 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..627 267174..267800 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004460
SUMMARY PARAGRAPH for YPT7

Rab proteins are small Ras-related GTPases that function in multiple stages in membrane traffic. Rabs continuously cycle between the cytosol and membranes. The GDP-bound form of the Rab is complexed with guanine nucleotide dissociation inhibitor(GDI) in the cytosol and are recruited to the membranes with the help of GDF (GDI Displacement Factor) and by their prenylation by geranylgeranyl transferase II (GGTase II). The membrane-bound Rab is activated to the GTP-bound form by the guanine nucleotide exchange factors (GEFs) which then goes on to interact with downstream effectors. The GTPase-activating protein (GAP) stimulates the hydrolysis of GTP to GDP to inactivate the Rab. Eleven Rabs have been identified in yeast and more than 60 in mammalian cells (reviewed in 8, 9, 10).

Last updated: 2010-05-18 Contact SGD

References cited on this page View Complete Literature Guide for YPT7
1) Wichmann H, et al.  (1992) Endocytosis in yeast: evidence for the involvement of a small GTP-binding protein (Ypt7p). Cell 71(7):1131-42
2) Wada Y, et al.  (1992) Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae. I. Isolation and characterization of two classes of vam mutants. J Biol Chem 267(26):18665-70
3) Schimmoller F and Riezman H  (1993) Involvement of Ypt7p, a small GTPase, in traffic from late endosome to the vacuole in yeast. J Cell Sci 106 ( Pt 3):823-30
4) Haas A, et al.  (1995) The GTPase Ypt7p of Saccharomyces cerevisiae is required on both partner vacuoles for the homotypic fusion step of vacuole inheritance. EMBO J 14(21):5258-70
5) Collins KM, et al.  (2005) Sec17p and HOPS, in distinct SNARE complexes, mediate SNARE complex disruption or assembly for fusion. EMBO J 24(10):1775-86
6) Liu TT, et al.  (2012) Rab GTPase regulation of retromer-mediated cargo export during endosome maturation. Mol Biol Cell 23(13):2505-15
7) Lazar T, et al.  (1997) Vesicular transport: how many Ypt/Rab-GTPases make a eukaryotic cell? Trends Biochem Sci 22(12):468-72
8) Cai H, et al.  (2007) Coats, tethers, Rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle. Dev Cell 12(5):671-82
9) Grosshans BL, et al.  (2006) Rabs and their effectors: achieving specificity in membrane traffic. Proc Natl Acad Sci U S A 103(32):11821-7
10) Bialek-Wyrzykowska U, et al.  (2000) Low levels of Ypt protein prenylation cause vesicle polarization defects and thermosensitive growth that can be suppressed by genes involved in cell wall maintenance. Mol Microbiol 35(6):1295-311