YPT6/YLR262C Summary Help

Standard Name YPT6 1
Systematic Name YLR262C
Feature Type ORF, Verified
Description Rab family GTPase; Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; resides temporarily at the Golgi, dissociates into cytosol upon arrival of the Rab GTPaseYpt32p, which also functions in the late Golgi; Golgi-localized form is bound to GTP, while cytosolic form is GDP-bound; homolog of the mammalian Rab6 (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description Yeast Protein Two 1
Chromosomal Location
ChrXII:668891 to 668244 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All YPT6 GO evidence and references
  View Computational GO annotations for YPT6
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
Resources
1417 total interaction(s) for 752 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 20
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 13
  • Biochemical Activity: 6
  • PCA: 3
  • Protein-peptide: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 14

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 2
  • Dosage Rescue: 19
  • Negative Genetic: 772
  • Phenotypic Enhancement: 15
  • Phenotypic Suppression: 5
  • Positive Genetic: 292
  • Synthetic Growth Defect: 95
  • Synthetic Lethality: 146
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 215
Molecular Weight (Da) 24,412
Isoelectric Point (pI) 5.58
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:668891 to 668244 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..648 668891..668244 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004252
SUMMARY PARAGRAPH for YPT6

Rab proteins are small Ras-related GTPases that function in multiple stages in membrane traffic. Rabs continuously cycle between the cytosol and membranes. The GDP-bound form of the Rab is complexed with guanine nucleotide dissociation inhibitor(GDI) in the cytosol and are recruited to the membranes with the help of GDF (GDI Displacement Factor) and by their prenylation by geranylgeranyl transferase II (GGTase II). The membrane-bound Rab is activated to the GTP-bound form by the guanine nucleotide exchange factors (GEFs) which then goes on to interact with downstream effectors. The GTPase-activating protein (GAP) stimulates the hydrolysis of GTP to GDP to inactivate the Rab. Eleven Rabs have been identified in yeast and more than 60 in mammalian cells (reviewed in 6, 7, 8).

Last updated: 2010-05-18 Contact SGD

References cited on this page View Complete Literature Guide for YPT6
1) Li B and Warner JR  (1996) Mutation of the Rab6 homologue of Saccharomyces cerevisiae, YPT6, inhibits both early Golgi function and ribosome biosynthesis. J Biol Chem 271(28):16813-9
2) Lazar T, et al.  (1997) Vesicular transport: how many Ypt/Rab-GTPases make a eukaryotic cell? Trends Biochem Sci 22(12):468-72
3) Luo Z and Gallwitz D  (2003) Biochemical and genetic evidence for the involvement of yeast Ypt6-GTPase in protein retrieval to different Golgi compartments. J Biol Chem 278(2):791-9
4) Suda Y, et al.  (2013) Rab GAP cascade regulates dynamics of Ypt6 in the Golgi traffic. Proc Natl Acad Sci U S A 110(47):18976-18981
5) Kawamura S, et al.  (2014) Analysis of subcellular localization and function of the yeast Rab6 homologue, Ypt6p, using a novel amino-terminal tagging strategy. Biochem Biophys Res Commun ()
6) Cai H, et al.  (2007) Coats, tethers, Rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle. Dev Cell 12(5):671-82
7) Grosshans BL, et al.  (2006) Rabs and their effectors: achieving specificity in membrane traffic. Proc Natl Acad Sci U S A 103(32):11821-7
8) Bialek-Wyrzykowska U, et al.  (2000) Low levels of Ypt protein prenylation cause vesicle polarization defects and thermosensitive growth that can be suppressed by genes involved in cell wall maintenance. Mol Microbiol 35(6):1295-311