GDB1/YPR184W Summary Help

GDB1 BASIC INFORMATION

Standard Name GDB1
Systematic Name YPR184W
Feature Type ORF, Verified
Description Glycogen debranching enzyme containing glucanotranferase and alpha-1,6-amyloglucosidase activities, required for glycogen degradation; phosphorylated in mitochondria (1, 2 and see Summary Paragraph)
GO Annotations All GDB1 GO evidence and references
    View Computational GO annotations for GDB1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Pathways
Mutant Phenotype All GDB1 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions GDB1 All interactions details and references
16 total interaction(s) for 15 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 2
  • PCA: 1
  • Two-hybrid: 5
Sequence Information
ChrXVI:902040 to 906650 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2004-07-21 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..4611 902040..906650 2004-07-21 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000006388

GDB1 RESOURCES

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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for GDB1

SUMMARY PARAGRAPH for GDB1

Glycogen is a branched polysaccharide of high molecular mass that is used as a storage carbohydrate. In S. cerevisiae, glycogen is typically catabolized to glucose-1-phosphate and glucose by the Gph1p glycogen phosphorylase and the Gdb1p debranching enzyme (3). However, during sporulation, glycogen is rapidly catabolized to glucose by the Sga1p glucoamylase (3).

The Gdb1p dual-activity glycogen debranching enzyme eliminates branchpoints in glycogen in a two-step process that includes (i) the transfer of a maltotriosyl (or maltosyl) unit from the branchpoint to an adjacent alpha-1,4-glucosyl chain using its oligo-1,4 to 1,4-glucanotransferase activity (EC 2.4.1.25), and (ii) the subsequent hydrolysis of the residual alpha-1,6-linked glucose residue by its alpha-1,6-glucosidase activity (EC 3.2.1.33) (1). Once the branch is resolved, glycogen degradation continues by the action of a second enzyme, the Gph1p glycogen phosphorylase (3).

GDB1 expression is regulated by stress-response elements, and is induced during late exponential growth phase and in response to various stresses, as are other glycogen metabolism genes (1). gdb1 null mutants are viable, but display increased accumulation of glycogen (1). No phenotypes corresponding to the mammalian glycogen storage disease III, associated with mutations in human glycogen debranching enzyme (AGL), have been identified for S. cerevisiae gdb1 null mutants (3).

Last updated: 2005-08-25

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for GDB1]

1) Teste MA, et al.  (2000) The Saccharomyces cerevisiae YPR184w gene encodes the glycogen debranching enzyme. FEMS Microbiol Lett 193(1):105-10
2) Reinders J, et al.  (2007) Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol Cell Proteomics 6(11):1896-906
3) Francois J and Parrou JL  (2001) Reserve carbohydrates metabolism in the yeast Saccharomyces cerevisiae. FEMS Microbiol Rev 25(1):125-45