DPB2/YPR175W Summary Help

Standard Name DPB2 1
Systematic Name YPR175W
Feature Type ORF, Verified
Description Second largest subunit of DNA polymerase II (DNA polymerase epsilon); required for maintenance of fidelity of chromosomal replication; essential motif in C-terminus is required for formation of the four-subunit Pol epsilon; expression peaks at the G1/S phase boundary; Cdc28p substrate (1, 2, 3, 4)
Name Description DNA Polymerase B subunit 2 1
Chromosomal Location
ChrXVI:888974 to 891043 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All DPB2 GO evidence and references
  View Computational GO annotations for DPB2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Resources
Classical genetics
conditional
null
Large-scale survey
null
reduction of function
repressible
Resources
126 total interaction(s) for 60 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 53
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 23
  • Biochemical Activity: 2
  • Co-crystal Structure: 1
  • Co-fractionation: 1
  • Co-purification: 4
  • Reconstituted Complex: 5
  • Two-hybrid: 20

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 2
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 2
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 689
Molecular Weight (Da) 78,339
Isoelectric Point (pI) 6.11
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:888974 to 891043 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2003-09-22
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2070 888974..891043 2011-02-03 2003-09-22
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006379
References cited on this page View Complete Literature Guide for DPB2
1) Araki H, et al.  (1991) DPB2, the gene encoding DNA polymerase II subunit B, is required for chromosome replication in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 88(11):4601-5
2) Jaszczur M, et al.  (2008) Dpb2p, a Noncatalytic Subunit of DNA Polymerase {varepsilon}, Contributes to the Fidelity of DNA Replication in Saccharomyces cerevisiae. Genetics 178(2):633-47
3) Jaszczur M, et al.  (2009) Defective interaction between Pol2p and Dpb2p, subunits of DNA polymerase epsilon, contributes to a mutator phenotype in Saccharomyces cerevisiae. Mutat Res 669(1-2):27-35
4) Isoz I, et al.  (2012) The C-terminus of Dpb2 is required for interaction with Pol2 and for cell viability. Nucleic Acids Res 40(22):11545-53