SGV1/YPR161C Summary Help

Standard Name SGV1 1
Systematic Name YPR161C
Alias BUR1 2
Feature Type ORF, Verified
Description Cyclin (Bur2p)-dependent protein kinase; part of the BUR kinase complex which functions in transcriptional regulation; phosphorylates the carboxy-terminal domain (CTD) of Rpo21p and the C-terminal repeat domain of Spt5p; recruits Spt6p to the CTD at the onset of transcription; regulated by Cak1p; similar to metazoan CDK9 proteins (2, 3, 4, 5, 6, 7, 8, 9)
Name Description Suppressor of Gpa1Val50 mutation 1
Chromosomal Location
ChrXVI:866422 to 864449 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SGV1 GO evidence and references
  View Computational GO annotations for SGV1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
conditional
null
overexpression
reduction of function
unspecified
Large-scale survey
conditional
null
overexpression
reduction of function
Resources
177 total interaction(s) for 135 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 86
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 11
  • Biochemical Activity: 9
  • Co-purification: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 4
  • Negative Genetic: 24
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 14
  • Synthetic Lethality: 10
  • Synthetic Rescue: 10

Resources
Expression Summary
histogram
Resources
Length (a.a.) 657
Molecular Weight (Da) 74,239
Isoelectric Point (pI) 10.36
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:866422 to 864449 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1974 866422..864449 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006365
References cited on this page View Complete Literature Guide for SGV1
1) Irie K, et al.  (1991) SGV1 encodes a CDC28/cdc2-related kinase required for a G alpha subunit-mediated adaptive response to pheromone in S. cerevisiae. Cell 65(5):785-95
2) Prelich G and Winston F  (1993) Mutations that suppress the deletion of an upstream activating sequence in yeast: involvement of a protein kinase and histone H3 in repressing transcription in vivo. Genetics 135(3):665-76
3) Yao S, et al.  (2000) BUR1 and BUR2 encode a divergent cyclin-dependent kinase-cyclin complex important for transcription in vivo. Mol Cell Biol 20(19):7080-7
4) Murray S, et al.  (2001) Phosphorylation of the RNA polymerase II carboxy-terminal domain by the Bur1 cyclin-dependent kinase. Mol Cell Biol 21(13):4089-96
5) Yao S and Prelich G  (2002) Activation of the Bur1-Bur2 cyclin-dependent kinase complex by Cak1. Mol Cell Biol 22(19):6750-8
6) Keogh MC, et al.  (2003) Bur1 kinase is required for efficient transcription elongation by RNA polymerase II. Mol Cell Biol 23(19):7005-18
7) Liu Y, et al.  (2009) Phosphorylation of the transcription elongation factor Spt5 by yeast Bur1 kinase stimulates recruitment of the PAF complex. Mol Cell Biol 29(17):4852-63
8) Bartkowiak B, et al.  (2010) CDK12 is a transcription elongation-associated CTD kinase, the metazoan ortholog of yeast Ctk1. Genes Dev 24(20):2303-16
9) Dronamraju R and Strahl BD  (2014) A feed forward circuit comprising Spt6, Ctk1 and PAF regulates Pol II CTD phosphorylation and transcription elongation. Nucleic Acids Res 42(2):870-81