Standard Name	PUF2
Systematic Name	YPR042C
Feature Type	ORF, Verified
Description	mRNA-binding protein; member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins; PUF2 has a paralog, JSN1, that arose from the whole genome duplication (1, 2)
Name Description	PUmilio-homology domain Family 3

Chromosomal Location	ChrXVI:653662 to 650435 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for PUF2
Molecular Function
Manually curated	mRNA binding (ISS)
High-throughput	mRNA binding (IDA)
Biological Process
Manually curated	nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay (IGI)
Cellular Component
High-throughput	cytoplasm (IDA)

Large-scale survey
null	competitive fitness: decreased resistance to streptomycin: decreased resistance to cycloheximide: decreased starvation resistance: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	38 total interaction(s) for 34 unique genes/features.
Physical Interactions	Affinity Capture-MS: 8 Affinity Capture-RNA: 3 Affinity Capture-Western: 1 Biochemical Activity: 2 PCA: 1 Protein-RNA: 3 Reconstituted Complex: 1 Two-hybrid: 1
Genetic Interactions	Negative Genetic: 9 Phenotypic Enhancement: 1 Phenotypic Suppression: 1 Positive Genetic: 6 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXVI:653662 to 650435 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..3228 653662..650435 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000006246