GCR1/YPL075W Summary Help

Standard Name GCR1 1
Systematic Name YPL075W
Alias LPF10
Feature Type ORF, Verified
Description Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p (2, 3 and see Summary Paragraph)
Name Description GlyColysis Regulation 1
Chromosomal Location
ChrXVI:412254 to 415362 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All GCR1 GO evidence and references
  View Computational GO annotations for GCR1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulatory Role
Binding motifs Predicted GCR1 Binding Site Locations
Resources
Mutant phenotypes All GCR1 Phenotype evidence and references
Classical genetics
null
Large-scale survey
null
reduction of function
Resources
interactions All GCR1 Interaction evidence and references
109 total interaction(s) for 87 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 6
  • Reconstituted Complex: 2
  • Two-hybrid: 11
Genetic Interactions
  • Dosage Rescue: 2
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 3
  • Synthetic Lethality: 72
  • Synthetic Rescue: 3
Resources
Expression Summary
histogram
Resources
Protein Information All GCR1 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:412254 to 415362 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-10-20
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..8 412254..412261 2011-02-03 1997-10-20
Intron 9..759 412262..413012 2011-02-03 1997-10-20
CDS 760..3109 413013..415362 2011-02-03 1997-10-20
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005996
SUMMARY PARAGRAPH for GCR1

Gcr1p and Gcr2p are transcriptional activators of glycolytic genes in Saccharomyces cerevisiae (4,5). Gcr1p is a DNA-binding protein interacting with the consensus sequence CTTCC, whereas Gcr2p interacts with Gcr1p (6,2). Both factors are needed for normal transcriptional activation (7,8). Null and point mutants have decreased levels of most of the glycolytic enzymes (9,3,10). The gcr1 mutant of yeast grows at near wild-type rates on nonfermentable carbon sources but exhibits a severe growth defect when grown in the presence of glucose, even when nonfermentable carbon sources are available (10). The human gene, hSGT1, can substitute for GCR2 but not GCR1 (11).

Last updated: 2005-07-21

References cited on this page View Complete Literature Guide for GCR1
1) Clifton D, et al.  (1978) Glycolysis mutants in Saccharomyces cerevisiae. Genetics 88(1):1-11
2) Uemura H and Jigami Y  (1992) Role of GCR2 in transcriptional activation of yeast glycolytic genes. Mol Cell Biol 12(9):3834-42
3) Holland MJ, et al.  (1987) The GCR1 gene encodes a positive transcriptional regulator of the enolase and glyceraldehyde-3-phosphate dehydrogenase gene families in Saccharomyces cerevisiae. Mol Cell Biol 7(2):813-20
4) Uemura H and Fraenkel DG  (1999) Glucose metabolism in gcr mutants of Saccharomyces cerevisiae. J Bacteriol 181(15):4719-23
5) Chambers A, et al.  (1995) Control of glycolytic gene expression in the budding yeast (Saccharomyces cerevisiae). Curr Genet 29(1):1-9
6) Baker HV  (1991) GCR1 of Saccharomyces cerevisiae encodes a DNA binding protein whose binding is abolished by mutations in the CTTCC sequence motif. Proc Natl Acad Sci U S A 88(21):9443-7
7) Huie MA and Baker HV  (1996) DNA-binding properties of the yeast transcriptional activator, Gcr1p. Yeast 12(4):307-17
8) Uemura H and Jigami Y  (1995) Mutations in GCR1, a transcriptional activator of Saccharomyces cerevisiae glycolytic genes, function as suppressors of gcr2 mutations. Genetics 139(2):511-21
9) Uemura H and Fraenkel DG  (1990) gcr2, a new mutation affecting glycolytic gene expression in Saccharomyces cerevisiae. Mol Cell Biol 10(12):6389-96
10) Lopez MC and Baker HV  (2000) Understanding the growth phenotype of the yeast gcr1 mutant in terms of global genomic expression patterns. J Bacteriol 182(17):4970-8
11) Sato T, et al.  (1999) A human gene, hSGT1, can substitute for GCR2, which encodes a general regulatory factor of glycolytic gene expression in Saccharomyces cerevisiae. Mol Gen Genet 260(6):535-40
12) Matys V, et al.  (2003) TRANSFAC: transcriptional regulation, from patterns to profiles. Nucleic Acids Res 31(1):374-8