GCR1/YPL075W Summary Help

Standard Name GCR1 1
Systematic Name YPL075W
Alias LPF10 , SIT3 2
Feature Type ORF, Verified
Description Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p (3, 4 and see Summary Paragraph)
Name Description GlyColysis Regulation 1
Chromosomal Location
ChrXVI:412254 to 415362 | ORF Map | GBrowse
Gene Ontology Annotations All GCR1 GO evidence and references
  View Computational GO annotations for GCR1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 685 genes
Regulators 13 genes
Classical genetics
Large-scale survey
reduction of function
110 total interaction(s) for 87 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 6
  • Reconstituted Complex: 2
  • Two-hybrid: 11

Genetic Interactions
  • Dosage Rescue: 2
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 3
  • Synthetic Lethality: 72
  • Synthetic Rescue: 3

Expression Summary
Length (a.a.) 785
Molecular Weight (Da) 87,728
Isoelectric Point (pI) 8.5
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:412254 to 415362 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1997-10-20
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..8 412254..412261 2011-02-03 1997-10-20
Intron 9..759 412262..413012 2011-02-03 1997-10-20
CDS 760..3109 413013..415362 2011-02-03 1997-10-20
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005996

Gcr1p and Gcr2p are transcriptional activators of glycolytic genes in Saccharomyces cerevisiae (5,6). Gcr1p is a DNA-binding protein interacting with the consensus sequence CTTCC, whereas Gcr2p interacts with Gcr1p (7,3). Both factors are needed for normal transcriptional activation (8,9). Null and point mutants have decreased levels of most of the glycolytic enzymes (10,4,11). The gcr1 mutant of yeast grows at near wild-type rates on nonfermentable carbon sources but exhibits a severe growth defect when grown in the presence of glucose, even when nonfermentable carbon sources are available (11). The human gene, hSGT1, can substitute for GCR2 but not GCR1 (12).

Last updated: 2005-07-21 Contact SGD

References cited on this page View Complete Literature Guide for GCR1
1) Clifton D, et al.  (1978) Glycolysis mutants in Saccharomyces cerevisiae. Genetics 88(1):1-11
2) Devlin C, et al.  (1991) RAP1 is required for BAS1/BAS2- and GCN4-dependent transcription of the yeast HIS4 gene. Mol Cell Biol 11(7):3642-51
3) Uemura H and Jigami Y  (1992) Role of GCR2 in transcriptional activation of yeast glycolytic genes. Mol Cell Biol 12(9):3834-42
4) Holland MJ, et al.  (1987) The GCR1 gene encodes a positive transcriptional regulator of the enolase and glyceraldehyde-3-phosphate dehydrogenase gene families in Saccharomyces cerevisiae. Mol Cell Biol 7(2):813-20
5) Uemura H and Fraenkel DG  (1999) Glucose metabolism in gcr mutants of Saccharomyces cerevisiae. J Bacteriol 181(15):4719-23
6) Chambers A, et al.  (1995) Control of glycolytic gene expression in the budding yeast (Saccharomyces cerevisiae). Curr Genet 29(1):1-9
7) Baker HV  (1991) GCR1 of Saccharomyces cerevisiae encodes a DNA binding protein whose binding is abolished by mutations in the CTTCC sequence motif. Proc Natl Acad Sci U S A 88(21):9443-7
8) Huie MA and Baker HV  (1996) DNA-binding properties of the yeast transcriptional activator, Gcr1p. Yeast 12(4):307-17
9) Uemura H and Jigami Y  (1995) Mutations in GCR1, a transcriptional activator of Saccharomyces cerevisiae glycolytic genes, function as suppressors of gcr2 mutations. Genetics 139(2):511-21
10) Uemura H and Fraenkel DG  (1990) gcr2, a new mutation affecting glycolytic gene expression in Saccharomyces cerevisiae. Mol Cell Biol 10(12):6389-96
11) Lopez MC and Baker HV  (2000) Understanding the growth phenotype of the yeast gcr1 mutant in terms of global genomic expression patterns. J Bacteriol 182(17):4970-8
12) Sato T, et al.  (1999) A human gene, hSGT1, can substitute for GCR2, which encodes a general regulatory factor of glycolytic gene expression in Saccharomyces cerevisiae. Mol Gen Genet 260(6):535-40
13) Matys V, et al.  (2003) TRANSFAC: transcriptional regulation, from patterns to profiles. Nucleic Acids Res 31(1):374-8