CHL1/YPL008W Summary Help

Standard Name CHL1 1
Systematic Name YPL008W
Alias CTF1 2 , LPA9 , MCM12 3
Feature Type ORF, Verified
Description Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome (4, 5, 6, 7, 8)
Name Description CHromosome Loss 9
Chromosomal Location
ChrXVI:539385 to 541970 | ORF Map | GBrowse
Genetic position: -6 cM
Gene Ontology Annotations All CHL1 GO evidence and references
  View Computational GO annotations for CHL1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 35 genes
Classical genetics
Large-scale survey
409 total interaction(s) for 246 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 7
  • Reconstituted Complex: 1
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 11
  • Dosage Rescue: 1
  • Negative Genetic: 175
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 1
  • Positive Genetic: 10
  • Synthetic Growth Defect: 51
  • Synthetic Lethality: 135

Expression Summary
Length (a.a.) 861
Molecular Weight (Da) 98,805
Isoelectric Point (pI) 7.91
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:539385 to 541970 | ORF Map | GBrowse
Genetic position: -6 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2586 539385..541970 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005929
References cited on this page View Complete Literature Guide for CHL1
1) Gerring SL, et al.  (1990) The CHL 1 (CTF 1) gene product of Saccharomyces cerevisiae is important for chromosome transmission and normal cell cycle progression in G2/M. EMBO J 9(13):4347-58
2) Spencer F, et al.  (1990) Mitotic chromosome transmission fidelity mutants in Saccharomyces cerevisiae. Genetics 124(2):237-49
3) Laha S, et al.  (2006) The budding yeast protein Chl1p is required to preserve genome integrity upon DNA damage in S-phase. Nucleic Acids Res 34(20):5880-91
4) Skibbens RV  (2004) Chl1p, a DNA helicase-like protein in budding yeast, functions in sister-chromatid cohesion. Genetics 166(1):33-42
5) Das SP and Sinha P  (2005) The budding yeast protein Chl1p has a role in transcriptional silencing, rDNA recombination, and aging. Biochem Biophys Res Commun 337(1):167-72
6) Wu Y, et al.  (2009) Welcome the family of FANCJ-like helicases to the block of genome stability maintenance proteins. Cell Mol Life Sci 66(7):1209-22
7) Wu Y, et al.  (2012) Biochemical characterization of Warsaw breakage syndrome helicase. J Biol Chem 287(2):1007-21
8) Ward TA, et al.  (2012) Components of a fanconi-like pathway control pso2-independent DNA interstrand crosslink repair in yeast. PLoS Genet 8(8):e1002884
9) Liras P, et al.  (1978) Characterization of a mutation in yeast causing nonrandom chromosome loss during mitosis. Genetics 88(4 Pt 1):651-71