VTS1/YOR359W Summary Help

Standard Name VTS1 1
Systematic Name YOR359W
Feature Type ORF, Verified
Description Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity (2, 3, 4, 5)
Name Description VTi1-2 Suppressor 1
Chromosomal Location
ChrXV:1011189 to 1012760 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All VTS1 GO evidence and references
  View Computational GO annotations for VTS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
repressible
Resources
35 total interaction(s) for 34 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 3
  • Biochemical Activity: 1
  • Protein-RNA: 1
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 8
  • Positive Genetic: 4
  • Synthetic Growth Defect: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 523
Molecular Weight (Da) 57,556
Isoelectric Point (pI) 9.59
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:1011189 to 1012760 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1572 1011189..1012760 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005886
References cited on this page View Complete Literature Guide for VTS1
1) Dilcher M, et al.  (2001) Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6. J Biol Chem 276(37):34537-44
2) Aviv T, et al.  (2003) The RNA-binding SAM domain of Smaug defines a new family of post-transcriptional regulators. Nat Struct Biol 10(8):614-21
3) Oberstrass FC, et al.  (2006) Shape-specific recognition in the structure of the Vts1p SAM domain with RNA. Nat Struct Mol Biol 13(2):160-7
4) Rendl LM, et al.  (2008) S. cerevisiae Vts1p induces deadenylation-dependent transcript degradation and interacts with the Ccr4p-Pop2p-Not deadenylase complex. RNA 14(7):1328-36
5) Lee CH, et al.  (2010) Involvement of Vts1, a structure-specific RNA-binding protein, in Okazaki fragment processing in yeast. Nucleic Acids Res 38(5):1583-95