MIP1/YOR330C Summary Help

Standard Name MIP1 1
Systematic Name YOR330C
Feature Type ORF, Verified
Description Mitochondrial DNA polymerase gamma subunit; conserved C-terminal segment is required for the maintenance of mitochondrial genome; its human ortholog, POLG, can rescue mip1 phenotypes, and mutations in POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit (1, 2, 3, 4, 5, 6, 7)
Name Description MItochondrial DNA Polymerase
Gene Product Alias mitochondrial DNA polymerase catalytic subunit 1
Chromosomal Location
ChrXV:943385 to 939621 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 181 cM
Gene Ontology Annotations All MIP1 GO evidence and references
  View Computational GO annotations for MIP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
dominant negative
gain of function
null
reduction of function
Large-scale survey
null
unspecified
Resources
187 total interaction(s) for 154 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-Western: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 117
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 6
  • Positive Genetic: 46
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,254
Molecular Weight (Da) 143,501
Isoelectric Point (pI) 9.1
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:943385 to 939621 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 181 cM
Last Update Coordinates: 2011-02-03 | Sequence: 2005-11-30
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3765 943385..939621 2011-02-03 2005-11-30
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005857
References cited on this page View Complete Literature Guide for MIP1
1) Foury F  (1989) Cloning and sequencing of the nuclear gene MIP1 encoding the catalytic subunit of the yeast mitochondrial DNA polymerase. J Biol Chem 264(34):20552-60
2) Lucas P, et al.  (2004) Absence of accessory subunit in the DNA polymerase gamma purified from yeast mitochondria. Mitochondrion 4(1):13-20
3) Stuart GR, et al.  (2006) Mitochondrial and nuclear DNA defects in Saccharomyces cerevisiae with mutations in DNA polymerase gamma associated with progressive external ophthalmoplegia. Hum Mol Genet 15(2):363-74
4) Young MJ, et al.  (2006) The carboxyl-terminal extension on fungal mitochondrial DNA polymerases: identification of a critical region of the enzyme from Saccharomyces cerevisiae. Yeast 23(2):101-16
5) Baruffini E, et al.  (2011) Predicting the contribution of novel POLG mutations to human disease through analysis in yeast model. Mitochondrion 11(1):182-90
6) Stewart JD, et al.  (2010) Polymerase ? gene POLG determines the risk of sodium valproate-induced liver toxicity. Hepatology 52(5):1791-6
7) Qian Y, et al.  (2014) Yeast Cells Expressing the Human Mitochondrial DNA Polymerase Reveal Correlations between Polymerase Fidelity and Human Disease Progression. J Biol Chem 289(9):5970-85