ABP140/YOR239W Summary Help

Standard Name ABP140 1
Systematic Name YOR239W
Alias YOR240W , TRM140 2 , 3
Feature Type ORF, Verified
Description AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift (1, 2, 3)
Name Description Actin Binding Protein
Chromosomal Location
ChrXV:784857 to 786744 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All ABP140 GO evidence and references
  View Computational GO annotations for ABP140
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Classical genetics
null
Large-scale survey
null
overexpression
unspecified
Resources
33 total interaction(s) for 32 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 2
  • Biochemical Activity: 11
  • Co-localization: 1
  • Reconstituted Complex: 1

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 8
  • Synthetic Growth Defect: 3
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 628
Molecular Weight (Da) 71,485
Isoelectric Point (pI) 4.44
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:784857 to 786744 | ORF Map | GBrowse
This feature contains embedded feature(s): YOR240W
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2000-08-14
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..831 784857..785687 2011-02-03 2000-08-14
Plus 1 translational frameshift 832..832 785688..785688 2011-02-03 2000-08-14
CDS 833..1888 785689..786744 2011-02-03 2000-08-14
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005765
References cited on this page View Complete Literature Guide for ABP140
1) Asakura T, et al.  (1998) Isolation and characterization of a novel actin filament-binding protein from Saccharomyces cerevisiae. Oncogene 16(1):121-30
2) Noma A, et al.  (2011) Actin-binding protein ABP140 is a methyltransferase for 3-methylcytidine at position 32 of tRNAs in Saccharomyces cerevisiae. RNA 17(6):1111-9
3) D'Silva S, et al.  (2011) A domain of the actin binding protein Abp140 is the yeast methyltransferase responsible for 3-methylcytidine modification in the tRNA anti-codon loop. RNA 17(6):1100-10