Standard Name	ABP140 1
Systematic Name	YOR239W
Alias	YOR240W , TRM140 2 , 3
Feature Type	ORF, Verified
Description	AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift (1, 2, 3)
Name Description	Actin Binding Protein

Chromosomal Location	ChrXV:784857 to 786744 | ORF Map | GBrowse

	View Computational GO annotations for ABP140
Molecular Function
Manually curated	actin filament binding (IDA) protein binding, bridging (IDA) tRNA (cytosine-3-)-methyltransferase activity (IDA, IMP)
Biological Process
Manually curated	actin filament bundle assembly (IDA) tRNA methylation (IMP)
Cellular Component
Manually curated	actin cortical patch (IDA) actin filament (IDA) actin filament bundle (IDA)
High-throughput	mating projection tip (IDA)

Classical genetics
null	tRNA-Thr modification: absent viable
Large-scale survey
null	competitive fitness: decreased competitive fitness: increased resistance to neomycin: decreased resistance to cycloheximide: decreased viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	31 total interaction(s) for 31 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-RNA: 2 Biochemical Activity: 11 Co-localization: 1 Reconstituted Complex: 1
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 1 Negative Genetic: 8 Synthetic Growth Defect: 3 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXV:784857 to 786744 | ORF Map | GBrowse
	This feature contains embedded feature(s): YOR240W
Last Update	Coordinates: 2011-02-03 | Sequence: 2000-08-14
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..831 784857..785687 2011-02-03 2000-08-14 Plus 1 translational frameshift 832..832 785688..785688 2011-02-03 2000-08-14 CDS 833..1888 785689..786744 2011-02-03 2000-08-14
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS) Plus_1_translational_frameshift (832 - 832)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005765