RFC1/YOR217W Summary Help

Standard Name RFC1 1
Systematic Name YOR217W
Alias CDC44 2
Feature Type ORF, Verified
Description Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon (1, 3)
Name Description Replication Factor C 1
Chromosomal Location
ChrXV:749301 to 751886 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RFC1 GO evidence and references
  View Computational GO annotations for RFC1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
conditional
Large-scale survey
null
reduction of function
Resources
99 total interaction(s) for 61 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 42
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 4
  • Co-purification: 4
  • FRET: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 15
  • Phenotypic Enhancement: 7
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 10
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 861
Molecular Weight (Da) 94,902
Isoelectric Point (pI) 9.99
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:749301 to 751886 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2586 749301..751886 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005743
References cited on this page View Complete Literature Guide for RFC1
1) Cullmann G, et al.  (1995) Characterization of the five replication factor C genes of Saccharomyces cerevisiae. Mol Cell Biol 15(9):4661-71
2) Moir D, et al.  (1982) Cold-sensitive cell-division-cycle mutants of yeast: isolation, properties, and pseudoreversion studies. Genetics 100(4):547-63
3) Yao N, et al.  (2003) Replication factor C clamp loader subunit arrangement within the circular pentamer and its attachment points to proliferating cell nuclear antigen. J Biol Chem 278(50):50744-53