MCA1/YOR197W Summary Help

Standard Name MCA1 1
Systematic Name YOR197W
Alias YCA1 2
Feature Type ORF, Verified
Description Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics and lifespan extension; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that block potential dimerization (2, 3, 4, 5, 6)
Name Description MetaCAspase 1
Chromosomal Location
ChrXV:717086 to 718384 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MCA1 GO evidence and references
  View Computational GO annotations for MCA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
62 total interaction(s) for 54 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 26
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 5
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 7
  • Synthetic Growth Defect: 1
  • Synthetic Rescue: 12

Resources
Expression Summary
histogram
Resources
Length (a.a.) 432
Molecular Weight (Da) 47,982
Isoelectric Point (pI) 5.09
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:717086 to 718384 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2006-05-11
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1299 717086..718384 2011-02-03 2006-05-11
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005723
References cited on this page View Complete Literature Guide for MCA1
1) Szallies A, et al.  (2002) A metacaspase of Trypanosoma brucei causes loss of respiration competence and clonal death in the yeast Saccharomyces cerevisiae. FEBS Lett 517(1-3):144-50
2) Madeo F, et al.  (2002) A caspase-related protease regulates apoptosis in yeast. Mol Cell 9(4):911-7
3) Lee RE, et al.  (2008) A non-death role of the yeast metacaspase: Yca1p alters cell cycle dynamics. PLoS One 3(8):e2956
4) Lee RE, et al.  (2010) Metacaspase Yca1 is required for clearance of insoluble protein aggregates. Proc Natl Acad Sci U S A 107(30):13348-53
5) Wong AH, et al.  (2012) Crystal structure of the yeast metacaspase Yca1. J Biol Chem 287(35):29251-9
6) Hill SM, et al.  (2014) Life-span extension by a metacaspase in the yeast Saccharomyces cerevisiae. Science 344(6190):1389-92