SLK19/YOR195W Summary Help

Standard Name SLK19 1
Systematic Name YOR195W
Feature Type ORF, Verified
Description Kinetochore-associated protein; required for chromosome segregation and kinetochore clustering; required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate (2, 3, 4, 5)
Name Description Synthetic Lethal Kar3p 1
Chromosomal Location
ChrXV:712866 to 715331 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SLK19 GO evidence and references
  View Computational GO annotations for SLK19
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
207 total interaction(s) for 112 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 6
  • Biochemical Activity: 2
  • PCA: 5
  • Two-hybrid: 32

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Lethality: 1
  • Negative Genetic: 68
  • Phenotypic Enhancement: 7
  • Phenotypic Suppression: 7
  • Positive Genetic: 5
  • Synthetic Growth Defect: 18
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 40
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 821
Molecular Weight (Da) 95,380
Isoelectric Point (pI) 4.65
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:712866 to 715331 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2466 712866..715331 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005721
References cited on this page View Complete Literature Guide for SLK19
1) Zeng X, et al.  (1999) Slk19p is a centromere protein that functions to stabilize mitotic spindles. J Cell Biol 146(2):415-25
2) Stegmeier F, et al.  (2002) Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function in a network that controls Cdc14 localization during early anaphase. Cell 108(2):207-20
3) Pfiz S, et al.  (2002) The yeast kinetochore protein Slk19 is required to prevent aberrant chromosome segregation in meiosis and mitosis. Genes Cells 7(10):1033-42
4) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
5) Richmond D, et al.  (2013) Slk19 clusters kinetochores and facilitates chromosome bipolar attachment. Mol Biol Cell 24(5):566-77