MED4/YOR174W Summary Help

Standard Name MED4 1
Systematic Name YOR174W
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation (1, 2)
Name Description MEDiator complex 1
Chromosomal Location
ChrXV:658747 to 659601 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MED4 GO evidence and references
  View Computational GO annotations for MED4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 1338 genes
Regulators 90 genes
Resources
Large-scale survey
conditional
null
reduction of function
unspecified
Resources
195 total interaction(s) for 94 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 75
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 40
  • Biochemical Activity: 1
  • Co-fractionation: 1
  • Co-purification: 14
  • Reconstituted Complex: 1
  • Two-hybrid: 17

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 37
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 284
Molecular Weight (Da) 32,205
Isoelectric Point (pI) 4.36
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:658747 to 659601 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..855 658747..659601 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005700
References cited on this page View Complete Literature Guide for MED4
1) Myers LC, et al.  (1998) The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Dev 12(1):45-54
2) Kornberg RD  (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9