EXO1/YOR033C Summary Help

Standard Name EXO1 1
Systematic Name YOR033C
Alias DHS1 2
Feature Type ORF, Verified
Description 5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair, MMS2 error-free branch of the post replication (PRR) pathway and DNA mismatch repair; role in telomere maintenance; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress; EXO1 has a paralog, DIN7, that arose from the whole genome duplication (3, 4, 5, 6)
Name Description EXOnuclease 1
Chromosomal Location
ChrXV:394524 to 392416 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All EXO1 GO evidence and references
  View Computational GO annotations for EXO1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
Large-scale survey
287 total interaction(s) for 102 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-Western: 7
  • Co-crystal Structure: 1
  • Co-purification: 1
  • Far Western: 2
  • Protein-peptide: 1
  • Two-hybrid: 12

Genetic Interactions
  • Dosage Rescue: 23
  • Negative Genetic: 63
  • Phenotypic Enhancement: 73
  • Phenotypic Suppression: 35
  • Positive Genetic: 2
  • Synthetic Growth Defect: 24
  • Synthetic Lethality: 18
  • Synthetic Rescue: 24

Expression Summary
Length (a.a.) 702
Molecular Weight (Da) 80,162
Isoelectric Point (pI) 8.35
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:394524 to 392416 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2109 394524..392416 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005559
References cited on this page View Complete Literature Guide for EXO1
1) Fiorentini P, et al.  (1997) Exonuclease I of Saccharomyces cerevisiae functions in mitotic recombination in vivo and in vitro. Mol Cell Biol 17(5):2764-73
2) Vodenicharov MD, et al.  (2010) Telomere capping in non-dividing yeast cells requires Yku and Rap1. EMBO J 29(17):3007-19
3) Tran PT, et al.  (2002) Characterization of nuclease-dependent functions of Exo1p in Saccharomyces cerevisiae. DNA Repair (Amst) 1(11):895-912
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Tran PT, et al.  (2007) A mutation in EXO1 defines separable roles in DNA mismatch repair and post-replication repair. DNA Repair (Amst) 6(11):1572-83
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76