FRE7/YOL152W Summary

Standard Name	FRE7
Systematic Name	YOL152W
Feature Type	ORF, Verified
Description	Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels (1, 2, 3 and see Summary Paragraph)
Name Description	Ferric REductase

Chromosomal Location	ChrXV:40748 to 42610 \| ORF Map \| GBrowse

Gene Ontology Annotations All FRE7 GO evidence and references

	View Computational GO annotations for FRE7
Molecular Function
Manually curated	ferric-chelate reductase activity (IGI)
Biological Process
Manually curated	copper ion import (IGI) iron ion transport (IGI)
Cellular Component
Manually curated	plasma membrane (IDA)
High-throughput	integral to membrane (ISM)

Mutant phenotypes All FRE7 Phenotype evidence and references

Classical genetics
null	viable
Large-scale survey
null	competitive fitness: decreased metal resistance: decreased oxidative stress resistance: decreased resistance to benzo[a]pyrene: decreased resistance to benzo[a]pyrene: increased resistance to ciclopirox olamine: decreased vacuolar morphology: abnormal viable
overexpression	resistance to sirolimus: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All FRE7 Interaction evidence and references

	25 total interaction(s) for 20 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1
Genetic Interactions	Dosage Rescue: 2 Negative Genetic: 19 Positive Genetic: 2 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All FRE7 Protein evidence and references

Localization	YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXV:40748 to 42610 | |

Last Update

Coordinates: 2011-02-03 | Sequence: 2006-01-05

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1863	40748..42610	2011-02-03	2006-01-05

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| TCDB \| UniProtKB

Primary SGDID	S000005512

SUMMARY PARAGRAPH for FRE7

FRE7 is part of a family of nine homologous genes involved or predicted to be involved in iron uptake that can be roughly grouped into three classes based on sequence similarity and transcriptional regulation: FRE1 and FRE7; FRE2 through FRE6; and FRE8 and YGL160W (1, 2). Mac1p induces transcription of FRE1 (2, 4, 5, 6, 7) and FRE7 (1, 2, 6) in response to low iron or copper levels. Deletion of FRE7 confers no apparent phenotype (8).

Fre1p and Fre2p are the major cell-surface iron reductases and together account for 90-98% of cell-surface reductase activity (9, 10, 11). Fre1p and Fre2p are homologous to the human gp91^phox protein (OMIM), the large subunit of human cytochrome b₅₅₈ (11, 12, 13), which reduces oxygen to bactericidal superoxide (O₂^-) on the surface of phagocytic leukocytes. Deficiency of gp91^phox causes X-linked chronic granulomatous disease (OMIM).

Last updated: 2005-08-17

References cited on this page View Complete Literature Guide for FRE7

1)	Martins LJ, et al. (1998) Metalloregulation of FRE1 and FRE2 homologs in Saccharomyces cerevisiae. J Biol Chem 273(37):23716-21
2)	Georgatsou E and Alexandraki D (1999) Regulated expression of the Saccharomyces cerevisiae Fre1p/Fre2p Fe/Cu reductase related genes. Yeast 15(7):573-84
3)	Yun CW, et al. (2001) The role of the FRE family of plasma membrane reductases in the uptake of siderophore-iron in Saccharomyces cerevisiae. J Biol Chem 276(13):10218-23
4)	Jungmann J, et al. (1993) MAC1, a nuclear regulatory protein related to Cu-dependent transcription factors is involved in Cu/Fe utilization and stress resistance in yeast. EMBO J 12(13):5051-6
5)	Yamaguchi-Iwai Y, et al. (1997) Homeostatic regulation of copper uptake in yeast via direct binding of MAC1 protein to upstream regulatory sequences of FRE1 and CTR1. J Biol Chem 272(28):17711-8
6)	Gross C, et al. (2000) Identification of the copper regulon in Saccharomyces cerevisiae by DNA microarrays. J Biol Chem 275(41):32310-6
7)	De Freitas JM, et al. (2004) Exploratory and confirmatory gene expression profiling of mac1Delta. J Biol Chem 279(6):4450-8
8)	Hajji K, et al. (1999) Disruption and phenotypic analysis of seven ORFs from the left arm of chromosome XV of Saccharomyces cerevisiae. Yeast 15(5):435-41
9)	Dancis A, et al. (1990) Genetic evidence that ferric reductase is required for iron uptake in Saccharomyces cerevisiae. Mol Cell Biol 10(5):2294-301
10)	Anderson GJ, et al. (1992) Ferric iron reduction and iron assimilation in Saccharomyces cerevisiae. J Inorg Biochem 47(3-4):249-55
11)	Georgatsou E and Alexandraki D (1994) Two distinctly regulated genes are required for ferric reduction, the first step of iron uptake in Saccharomyces cerevisiae. Mol Cell Biol 14(5):3065-73
12)	Dancis A, et al. (1992) Ferric reductase of Saccharomyces cerevisiae: molecular characterization, role in iron uptake, and transcriptional control by iron. Proc Natl Acad Sci U S A 89(9):3869-73
13)	Shatwell KP, et al. (1996) The FRE1 ferric reductase of Saccharomyces cerevisiae is a cytochrome b similar to that of NADPH oxidase. J Biol Chem 271(24):14240-4