PPM2/YOL141W Summary Help

Standard Name PPM2 1
Systematic Name YOL141W
Alias TYW4 2
Feature Type ORF, Verified
Description AdoMet-dependent tRNA methyltransferase; also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p (2, 3, 4, 5, 6)
Name Description Protein Phosphatase Methyltransferase 1
Chromosomal Location
ChrXV:56452 to 58539 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PPM2 GO evidence and references
  View Computational GO annotations for PPM2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 6 genes
Resources
Large-scale survey
null
overexpression
Resources
9 total interaction(s) for 9 unique genes/features.
Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 5
  • Positive Genetic: 2
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 695
Molecular Weight (Da) 78,956
Isoelectric Point (pI) 7.04
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:56452 to 58539 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2088 56452..58539 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005501
References cited on this page View Complete Literature Guide for PPM2
1) Wu J, et al.  (2000) Carboxyl methylation of the phosphoprotein phosphatase 2A catalytic subunit promotes its functional association with regulatory subunits in vivo. EMBO J 19(21):5672-81
2) Noma A, et al.  (2006) Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA. EMBO J 25(10):2142-54
3) Wei H, et al.  (2001) Carboxymethylation of the PP2A catalytic subunit in Saccharomyces cerevisiae is required for efficient interaction with the B-type subunits Cdc55p and Rts1p. J Biol Chem 276(2):1570-7
4) Kalhor HR, et al.  (2001) Protein phosphatase methyltransferase 1 (Ppm1p) is the sole activity responsible for modification of the major forms of protein phosphatase 2A in yeast. Arch Biochem Biophys 395(2):239-45
5) Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54
6) Noma A and Suzuki T  (2006) Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis. Nucleic Acids Symp Ser (Oxf) (50):65-6