ARG8/YOL140W Summary Help

Standard Name ARG8
Systematic Name YOL140W
Feature Type ORF, Verified
Description Acetylornithine aminotransferase; catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine (1 and see Summary Paragraph)
Name Description ARGinine requiring
Chromosomal Location
ChrXV:58759 to 60030 | ORF Map | GBrowse
Genetic position: -130 cM
Gene Ontology Annotations All ARG8 GO evidence and references
  View Computational GO annotations for ARG8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 9 genes
Large-scale survey
7 total interaction(s) for 7 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 2
  • Two-hybrid: 1

Genetic Interactions
  • Synthetic Lethality: 3

Expression Summary
Length (a.a.) 423
Molecular Weight (Da) 46,681
Isoelectric Point (pI) 7.55
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:58759 to 60030 | ORF Map | GBrowse
Genetic position: -130 cM
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1272 58759..60030 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005500

ARG8 encodes acetylornithine aminotransferase, a mitochondrial matrix enzyme that catalyzes the fourth step in the biosynthesis of ornithine (2), an intermediate in arginine biosynthesis. Arg8p is 68% identical to the acetylornithine aminotransferase from Kluyveromyces lactis, and the K. lactis gene can complement an S. cerevisiae arg8 mutant (3). Arg8p is also similar to E. coli ArgD (1). Like other genes encoding arginine biosynthetic enzymes, ARG8 is transcriptionally repressed in the presence of arginine and is regulated by general amino acid control (4, 5). Arginine-responsive transcription factors, including Arg80p, Arg81p, Arg82p, and Mcm1p, and their target upstream activating sequences in ARG8, have been identified (4, 6, 7, 5). The ARG8 sequence has been recoded using the mitochondrial genetic code, for use as a marker for mitochondrial transformation (8). A construct in which the recoded ARG8 replaces the COX3 coding sequence complements a deletion of the nuclear ARG8 gene, and its expression requires COX3 mRNA-specific translational activators (8).

Last updated: 1999-10-19 Contact SGD

References cited on this page View Complete Literature Guide for ARG8
1) Heimberg H, et al.  (1990) Escherichia coli and Saccharomyces cerevisiae acetylornithine aminotransferase: evolutionary relationship with ornithine aminotransferase. Gene 90(1):69-78
2) Jauniaux JC, et al.  (1978) Arginine metabolism in Saccharomyces cerevisiae: subcellular localization of the enzymes. J Bacteriol 133(3):1096-1107
3) Janssen A and Chen XJ  (1998) Cloning, sequencing and disruption of the ARG8 gene encoding acetylornithine aminotransferase in the petite-negative yeast Kluyveromyces lactis. Yeast 14(3):281-5
4) Messenguy F  (1987) Multiplicity of regulatory mechanisms controlling amino acid biosynthesis in Saccharomyces cerevisiae. Microbiol Sci 4(5):150-3
5) Hinnebusch A  (1992) "General and Pathway-specific Regulatory Mechanisms Controlling the Synthesis of Amino Acid Biosynthetic Enzymes in Saccharomyces cerevisiae". Pp. 319-414 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
6) Messenguy F and Dubois E  (1993) Genetic evidence for a role for MCM1 in the regulation of arginine metabolism in Saccharomyces cerevisiae. Mol Cell Biol 13(4):2586-92
7) Crabeel M, et al.  (1995) Further definition of the sequence and position requirements of the arginine control element that mediates repression and induction by arginine in Saccharomyces cerevisiae. Yeast 11(14):1367-80
8) Steele DF, et al.  (1996) Expression of a recoded nuclear gene inserted into yeast mitochondrial DNA is limited by mRNA-specific translational activation. Proc Natl Acad Sci U S A 93(11):5253-7