CDC33/YOL139C Summary Help

Standard Name CDC33
Systematic Name YOL139C
Alias TIF45
Feature Type ORF, Verified
Description mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth (1, 2, 3)
Name Description Cell Division Cycle
Gene Product Alias eIF4E
Chromosomal Location
ChrXV:61024 to 60383 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All CDC33 GO evidence and references
  View Computational GO annotations for CDC33
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
conditional
Large-scale survey
null
overexpression
reduction of function
repressible
unspecified
Resources
199 total interaction(s) for 120 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 119
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 20
  • Biochemical Activity: 8
  • Co-fractionation: 1
  • Far Western: 1
  • PCA: 2
  • Reconstituted Complex: 5
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 8
  • Negative Genetic: 1
  • Phenotypic Suppression: 2
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 15
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 213
Molecular Weight (Da) 24,254
Isoelectric Point (pI) 5.25
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:61024 to 60383 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..642 61024..60383 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005499
References cited on this page View Complete Literature Guide for CDC33
1) Fortes P, et al.  (2000) The yeast nuclear cap binding complex can interact with translation factor eIF4G and mediate translation initiation. Mol Cell 6(1):191-6
2) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
3) Ross D, et al.  (2012) eIF4E Is an Important Determinant of Adhesion and Pseudohyphal Growth of the Yeast S. cerevisiae. PLoS One 7(11):e50773