RFC4/YOL094C Summary Help

Standard Name RFC4 1
Systematic Name YOL094C
Feature Type ORF, Verified
Description Subunit of heteropentameric Replication factor C (RF-C); which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; relocalizes to the cytosol in response to hypoxia (1, 2, 3)
Name Description Replication Factor C 1
Chromosomal Location
ChrXV:142555 to 141584 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RFC4 GO evidence and references
  View Computational GO annotations for RFC4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
conditional
unspecified
Large-scale survey
conditional
null
reduction of function
repressible
Resources
138 total interaction(s) for 95 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 47
  • Affinity Capture-Western: 13
  • Co-fractionation: 1
  • Co-purification: 6
  • FRET: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 61
  • Phenotypic Enhancement: 1
  • Positive Genetic: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 323
Molecular Weight (Da) 36,149
Isoelectric Point (pI) 9.84
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:142555 to 141584 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..972 142555..141584 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005454
References cited on this page View Complete Literature Guide for RFC4
1) Cullmann G, et al.  (1995) Characterization of the five replication factor C genes of Saccharomyces cerevisiae. Mol Cell Biol 15(9):4661-71
2) Yao N, et al.  (2003) Replication factor C clamp loader subunit arrangement within the circular pentamer and its attachment points to proliferating cell nuclear antigen. J Biol Chem 278(50):50744-53
3) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30