PSH1/YOL054W Summary Help

Standard Name PSH1 1
Systematic Name YOL054W
Feature Type ORF, Verified
Description E3 ubiquitin ligase targeting centromere-binding protein Cse4p; mediates poyubiquitination and degradation of Cse4p; prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p (2, 3)
Name Description Pob3/Spt16 Histone associated
Chromosomal Location
ChrXV:228614 to 229834 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PSH1 GO evidence and references
  View Computational GO annotations for PSH1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
182 total interaction(s) for 110 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 80
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 5
  • Biochemical Activity: 4
  • Co-purification: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 4
  • Dosage Lethality: 4
  • Dosage Rescue: 3
  • Negative Genetic: 61
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 3
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 406
Molecular Weight (Da) 47,068
Isoelectric Point (pI) 4.09
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:228614 to 229834 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1221 228614..229834 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005415
References cited on this page View Complete Literature Guide for PSH1
1) Li AM, et al.  (2003) Scp160p associates with specific mRNAs in yeast. Nucleic Acids Res 31(7):1830-7
2) Ranjitkar P, et al.  (2010) An E3 Ubiquitin Ligase Prevents Ectopic Localization of the Centromeric Histone H3 Variant via the Centromere Targeting Domain. Mol Cell 40(3):455-64
3) Hewawasam G, et al.  (2010) Psh1 Is an E3 Ubiquitin Ligase that Targets the Centromeric Histone Variant Cse4. Mol Cell 40(3):444-54