LAG2/YOL025W Summary Help

Standard Name LAG2 1
Systematic Name YOL025W
Alias ECM36
Feature Type ORF, Verified
Description Protein that negatively regulates the SCF E3-ubiquitin ligase; regulates by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 (1, 2, 3)
Name Description Longevity Assurance Gene 4
Chromosomal Location
ChrXV:274957 to 276939 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All LAG2 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 6 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
unspecified
Resources
44 total interaction(s) for 20 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-Western: 7
  • Co-purification: 3
  • Reconstituted Complex: 8
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Growth Defect: 3
  • Negative Genetic: 6
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 1
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 660
Molecular Weight (Da) 75,638
Isoelectric Point (pI) 4.44
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:274957 to 276939 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-07-27
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1983 274957..276939 2011-02-03 1997-07-27
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005385
References cited on this page View Complete Literature Guide for LAG2
1) Childress AM, et al.  (1996) LAG2, a gene that determines yeast longevity. Microbiology 142 ( Pt 8):2289-97
2) Liu Y, et al.  (2009) A longevity protein, Lag2, interacts with SCF complex and regulates SCF function. EMBO J 28(21):3366-77
3) Siergiejuk E, et al.  (2009) Cullin neddylation and substrate-adaptors counteract SCF inhibition by the CAND1-like protein Lag2 in Saccharomyces cerevisiae. EMBO J 28(24):3845-56
4) D'mello NP, et al.  (1994) Cloning and characterization of LAG1, a longevity-assurance gene in yeast. J Biol Chem 269(22):15451-9