SIN3/YOL004W Summary Help

Standard Name SIN3 1
Systematic Name YOL004W
Alias CPE1 2 , GAM2 3 , RPD1 4 , 5 , SDI1 4 , 6 , SDS16 7 , 8 , UME4 4 , 9
Feature Type ORF, Verified
Description Component of both the Rpd3S and Rpd3L histone deacetylase complexes; involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity (10, 11, 12 and see Summary Paragraph)
Name Description Switch INdependent
Chromosomal Location
ChrXV:316938 to 321548 | ORF Map | GBrowse
Gbrowse
Genetic position: 1 cM
Gene Ontology Annotations All SIN3 GO evidence and references
  View Computational GO annotations for SIN3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Targets 2031 genes
Regulators 73 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
1016 total interaction(s) for 533 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 146
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 16
  • Biochemical Activity: 2
  • Co-purification: 2
  • PCA: 1
  • Reconstituted Complex: 9
  • Two-hybrid: 16

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 518
  • Phenotypic Enhancement: 24
  • Phenotypic Suppression: 18
  • Positive Genetic: 87
  • Synthetic Growth Defect: 85
  • Synthetic Lethality: 61
  • Synthetic Rescue: 26

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,536
Molecular Weight (Da) 174,838
Isoelectric Point (pI) 5.42
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:316938 to 321548 | ORF Map | GBrowse
SGD ORF map
Genetic position: 1 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4611 316938..321548 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005364
SUMMARY PARAGRAPH for SIN3

Sin3p is involved modifying chromatin structure through its interactions with the Rpd3p histone deacetylase complex, DNA-binding transcription factors, and other regulatory proteins. As a consequence, SIN3 is required for transcriptional regulation, the regulation of heterochromatin, and maintaining genome integrity (reviewed in 11).

Sin3p is found in a large multi-subunit complex that contains Rpd3p, a histone deacetylase (13, 14, 15). The Sin3p-Rpd3p histone deactylase complex is targeted to specific promoters via additional interactions between Sin3p and DNA-binding transcription factors, such as Ume6p (16). Once recruited to the promoter, the Sin3p-Rpd3p histone deacetylase complex generates localized regions of histone deacetylation, which results in transcriptional repression (17). Although SIN3 and RPD3 have been implicated in positively regulating gene expression (3, 4, 10, 18), the mechanism of transcriptional activation via hypo-acetylated histones is currently unknown. In addition to its role in transcriptional regulation, SIN3 is also required for silencing, maintaining telomere length, and repairing double-strand breaks (19, 20, 21).

Sin3p contains four paired amphipathic alpha helicies (PAH1-4) (22), a histone deacetylase interaction domain (HID) between PAH3 and PAH4, and a highly conserved region (HCR) at the C-terminal end (23). These domains are important for its repressor function and highly conserved in most eukaryotes (23, 24, 25, 11).

Last updated: 2006-08-11 Contact SGD

References cited on this page View Complete Literature Guide for SIN3
1) Sternberg PW, et al.  (1987) Activation of the yeast HO gene by release from multiple negative controls. Cell 48(4):567-77
2) Hudak KA, et al.  (1994) A pleiotropic phospholipid biosynthetic regulatory mutation in Saccharomyces cerevisiae is allelic to sin3 (sdi1, ume4, rpd1). Genetics 136(2):475-83
3) Yoshimoto H, et al.  (1992) The Saccharomyces cerevisiae GAM2/SIN3 protein plays a role in both activation and repression of transcription. Mol Gen Genet 233(1-2):327-30
4) Vidal M, et al.  (1991) RPD1 (SIN3/UME4) is required for maximal activation and repression of diverse yeast genes. Mol Cell Biol 11(12):6306-16
5) Vidal M, et al.  (1990) Direct selection for mutants with increased K+ transport in Saccharomyces cerevisiae. Genetics 125(2):313-20
6) Nasmyth K, et al.  (1987) Both positive and negative regulators of HO transcription are required for mother-cell-specific mating-type switching in yeast. Cell 48(4):579-87
7) Vannier D, et al.  (1996) Evidence that the transcriptional regulators SIN3 and RPD3, and a novel gene (SDS3) with similar functions, are involved in transcriptional silencing in S. cerevisiae. Genetics 144(4):1343-53
8) Sussel L, et al.  (1995) Suppressors of defective silencing in yeast: effects on transcriptional repression at the HMR locus, cell growth and telomere structure. Genetics 141(3):873-88
9) Strich R, et al.  (1989) Identification of negative regulatory genes that govern the expression of early meiotic genes in yeast. Proc Natl Acad Sci U S A 86(24):10018-22
10) Bernstein BE, et al.  (2000) Genomewide studies of histone deacetylase function in yeast. Proc Natl Acad Sci U S A 97(25):13708-13
11) Silverstein RA and Ekwall K  (2005) Sin3: a flexible regulator of global gene expression and genome stability. Curr Genet 47(1):1-17
12) Carrozza MJ, et al.  (2005) Stable incorporation of sequence specific repressors Ash1 and Ume6 into the Rpd3L complex. Biochim Biophys Acta 1731(2):77-87; discussion 75-6
13) Kasten MM and Stillman DJ  (1997) Identification of the Saccharomyces cerevisiae genes STB1-STB5 encoding Sin3p binding proteins. Mol Gen Genet 256(4):376-86
14) Kasten MM, et al.  (1997) A large protein complex containing the yeast Sin3p and Rpd3p transcriptional regulators. Mol Cell Biol 17(8):4852-8
15) Lechner T, et al.  (2000) Sds3 (suppressor of defective silencing 3) is an integral component of the yeast Sin3[middle dot]Rpd3 histone deacetylase complex and is required for histone deacetylase activity. J Biol Chem 275(52):40961-6
16) Rundlett SE, et al.  (1998) Transcriptional repression by UME6 involves deacetylation of lysine 5 of histone H4 by RPD3. Nature 392(6678):831-5
17) Kadosh D and Struhl K  (1998) Targeted recruitment of the Sin3-Rpd3 histone deacetylase complex generates a highly localized domain of repressed chromatin in vivo. Mol Cell Biol 18(9):5121-7
18) De Nadal E, et al.  (2004) The MAPK Hog1 recruits Rpd3 histone deacetylase to activate osmoresponsive genes. Nature 427(6972):370-4
19) Sun ZW and Hampsey M  (1999) A general requirement for the Sin3-Rpd3 histone deacetylase complex in regulating silencing in Saccharomyces cerevisiae. Genetics 152(3):921-32
20) Askree SH, et al.  (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
21) Yu Y, et al.  (2000) Architectural transcription factors and the SAGA complex function in parallel pathways to activate transcription. Mol Cell Biol 20(7):2350-7
22) Wang H, et al.  (1990) The Saccharomyces cerevisiae SIN3 gene, a negative regulator of HO, contains four paired amphipathic helix motifs. Mol Cell Biol 10(11):5927-36
23) Wang H and Stillman DJ  (1993) Transcriptional repression in Saccharomyces cerevisiae by a SIN3-LexA fusion protein. Mol Cell Biol 13(3):1805-14
24) Ayer DE, et al.  (1995) Mad-Max transcriptional repression is mediated by ternary complex formation with mammalian homologs of yeast repressor Sin3. Cell 80(5):767-76
25) Halleck MS, et al.  (1995) A widely distributed putative mammalian transcriptional regulator containing multiple paired amphipathic helices, with similarity to yeast SIN3. Genomics 26(2):403-6