YPT11/YNL304W Summary

Standard Name	YPT11 1 (see Nomenclature conflict Note)
Systematic Name	YNL304W
Feature Type	ORF, Verified
Description	Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells (1, 2, 3, 4 and see Summary Paragraph)
Name Description	Yeast Protein Two 1

Chromosomal Location	ChrXIV:60297 to 61550 \| ORF Map \| GBrowse

Gene Ontology Annotations All YPT11 GO evidence and references

	View Computational GO annotations for YPT11
Molecular Function
Manually curated	GTPase activity (IPI)
Biological Process
Manually curated	endoplasmic reticulum inheritance (IMP) Golgi inheritance (IGI, IMP) Golgi localization (IGI, IMP) mitochondrion inheritance (IGI, IMP)
Cellular Component
Manually curated	cellular bud neck (IDA) cellular bud tip (IDA) endoplasmic reticulum (IDA) incipient cellular bud site (IDA)

Mutant phenotypes All YPT11 Phenotype evidence and references

Classical genetics
overexpression	budding index: abnormal cell cycle progression in G2 phase: abnormal cell size: increased cellular morphology: abnormal nuclear morphology: abnormal
Large-scale survey
null	competitive fitness: decreased hyperosmotic stress resistance: decreased resistance to amitrole: decreased resistance to cycloheximide: decreased resistance to tunicamycin: decreased viable
overexpression	resistance to sirolimus: decreased vegetative growth: decreased rate vegetative growth: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All YPT11 Interaction evidence and references

	39 total interaction(s) for 27 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-Western: 3 Biochemical Activity: 3 Protein-peptide: 3 Reconstituted Complex: 3 Two-hybrid: 7
Genetic Interactions	Negative Genetic: 5 Phenotypic Suppression: 2 Synthetic Growth Defect: 4 Synthetic Lethality: 5 Synthetic Rescue: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All YPT11 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXIV:60297 to 61550 | |

Last Update

Coordinates: 2005-11-07 | Sequence: 2005-11-07

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1254	60297..61550	2005-11-07	2005-11-07

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005248

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
YPT32	Nomenclature conflict	Both YPT32/YGL210W, a GTPase involved in exocytosis and vesicle-mediated transport, and YPT11/YNL304W, a RAB small monomeric GTPase involved in mitochondrial inheritance, have been referred to as YPT11.

SUMMARY PARAGRAPH for YPT11

Rab proteins are small Ras-related GTPases that function in multiple stages in membrane traffic. Rabs continuously cycle between the cytosol and membranes. The GDP-bound form of the Rab is complexed with in the cytosol and are recruited to the membranes with the help of GDF (GDI Displacement Factor) and by their prenylation by geranylgeranyl transferase II (GGTase II). The membrane-bound Rab is activated to the GTP-bound form by the guanine nucleotide exchange factors (GEFs) which then goes on to interact with downstream effectors. The GTPase-activating protein (GAP) stimulates the hydrolysis of GTP to GDP to inactivate the Rab. Eleven Rabs have been identified in yeast and more than 60 in mammalian cells (reviewed in 5, 6, 7).

Last updated: 2010-05-18

References cited on this page View Complete Literature Guide for YPT11

1)	Lazar T, et al. (1997) Vesicular transport: how many Ypt/Rab-GTPases make a eukaryotic cell? Trends Biochem Sci 22(12):468-72
2)	Itoh T, et al. (2002) Complex formation with Ypt11p, a rab-type small GTPase, is essential to facilitate the function of Myo2p, a class V myosin, in mitochondrial distribution in Saccharomyces cerevisiae. Mol Cell Biol 22(22):7744-57
3)	Itoh T, et al. (2004) Mmr1p is a mitochondrial factor for Myo2p-dependent inheritance of mitochondria in the budding yeast. EMBO J 23(13):2520-30
4)	Buvelot Frei S, et al. (2006) Bioinformatic and comparative localization of Rab proteins reveals functional insights into the uncharacterized GTPases Ypt10p and Ypt11p. Mol Cell Biol 26(19):7299-317
5)	Cai H, et al. (2007) Coats, tethers, Rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle. Dev Cell 12(5):671-82
6)	Grosshans BL, et al. (2006) Rabs and their effectors: achieving specificity in membrane traffic. Proc Natl Acad Sci U S A 103(32):11821-7
7)	Bialek-Wyrzykowska U, et al. (2000) Low levels of Ypt protein prenylation cause vesicle polarization defects and thermosensitive growth that can be suppressed by genes involved in cell wall maintenance. Mol Microbiol 35(6):1295-311