RFC3/YNL290W Summary Help

Standard Name RFC3 1
Systematic Name YNL290W
Feature Type ORF, Verified
Description Subunit of heteropentameric Replication factor C (RF-C); which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; relocalizes to the cytosol in response to hypoxia (1, 2, 3)
Name Description Replication Factor C 1
Chromosomal Location
ChrXIV:86218 to 87240 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RFC3 GO evidence and references
  View Computational GO annotations for RFC3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Large-scale survey
null
reduction of function
Resources
134 total interaction(s) for 84 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 48
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 14
  • Co-fractionation: 1
  • Co-purification: 6
  • FRET: 1
  • PCA: 3
  • Reconstituted Complex: 6
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 45
  • Positive Genetic: 2
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 340
Molecular Weight (Da) 38,203
Isoelectric Point (pI) 6.4
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:86218 to 87240 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1023 86218..87240 2005-11-07 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005234
References cited on this page View Complete Literature Guide for RFC3
1) Cullmann G, et al.  (1995) Characterization of the five replication factor C genes of Saccharomyces cerevisiae. Mol Cell Biol 15(9):4661-71
2) Yao N, et al.  (2003) Replication factor C clamp loader subunit arrangement within the circular pentamer and its attachment points to proliferating cell nuclear antigen. J Biol Chem 278(50):50744-53
3) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30