SLA2/YNL243W Summary Help

Standard Name SLA2 1
Systematic Name YNL243W
Alias END4 2 , 3 , MOP2 4
Feature Type ORF, Verified
Description Adaptor protein that links actin to clathrin and endocytosis; involved in membrane cytoskeleton assembly and cell polarization; present in the actin cortical patch of the emerging bud tip; dimer in vivo (5, 6, 7, 8, 9)
Name Description Synthetic Lethal with ABP1 1
Chromosomal Location
ChrXIV:188051 to 190957 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SLA2 GO evidence and references
  View Computational GO annotations for SLA2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
conditional
null
Large-scale survey
null
Resources
157 total interaction(s) for 107 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 19
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 6
  • Biochemical Activity: 1
  • Co-fractionation: 3
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 12
  • Protein-peptide: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 32

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Phenotypic Enhancement: 12
  • Phenotypic Suppression: 6
  • Synthetic Growth Defect: 11
  • Synthetic Lethality: 44

Resources
Expression Summary
histogram
Resources
Length (a.a.) 968
Molecular Weight (Da) 108,911
Isoelectric Point (pI) 5.11
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:188051 to 190957 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2907 188051..190957 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005187
References cited on this page View Complete Literature Guide for SLA2
1) Holtzman DA, et al.  (1993) Synthetic-lethal interactions identify two novel genes, SLA1 and SLA2, that control membrane cytoskeleton assembly in Saccharomyces cerevisiae. J Cell Biol 122(3):635-44
2) Wesp A, et al.  (1997) End4p/Sla2p interacts with actin-associated proteins for endocytosis in Saccharomyces cerevisiae. Mol Biol Cell 8(11):2291-306
3) Raths S, et al.  (1993) end3 and end4: two mutants defective in receptor-mediated and fluid-phase endocytosis in Saccharomyces cerevisiae. J Cell Biol 120(1):55-65
4) Na S, et al.  (1995) MOP2 (SLA2) affects the abundance of the plasma membrane H(+)-ATPase of Saccharomyces cerevisiae. J Biol Chem 270(12):6815-23
5) Pruyne D and Bretscher A  (2000) Polarization of cell growth in yeast. J Cell Sci 113 ( Pt 4):571-85
6) Yang S, et al.  (1999) Sla2p is associated with the yeast cortical actin cytoskeleton via redundant localization signals. Mol Biol Cell 10(7):2265-83
7) Munn AL  (2001) Molecular requirements for the internalisation step of endocytosis: insights from yeast. Biochim Biophys Acta 1535(3):236-57
8) Palecek J, et al.  (2001) Rpg1p/Tif32p, a subunit of translation initiation factor 3, interacts with actin-associated protein Sla2p. Biochem Biophys Res Commun 282(5):1244-50
9) Baggett JJ, et al.  (2003) The Sla2p talin domain plays a role in endocytosis in Saccharomyces cerevisiae. Genetics 165(4):1661-74