LAP3/YNL239W Summary Help

Standard Name LAP3 1
Systematic Name YNL239W
Alias BLH1 2 , GAL6 3 , YCP1 4
Feature Type ORF, Verified
Description Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH (3, 4, 5, 6, 7)
Name Description Leucine AminoPeptidases 1
Chromosomal Location
ChrXIV:200569 to 201933 | ORF Map | GBrowse
Gbrowse
Genetic position: -152 cM
Gene Ontology Annotations All LAP3 GO evidence and references
  View Computational GO annotations for LAP3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
20 total interaction(s) for 17 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 1
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 7
  • Synthetic Growth Defect: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 454
Molecular Weight (Da) 52,088
Isoelectric Point (pI) 7.97
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:200569 to 201933 | ORF Map | GBrowse
SGD ORF map
Genetic position: -152 cM
Last Update Coordinates: 2011-02-03 | Sequence: 2003-01-07
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1365 200569..201933 2011-02-03 2003-01-07
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005183
References cited on this page View Complete Literature Guide for LAP3
1) Trumbly RJ and Bradley G  (1983) Isolation and characterization of aminopeptidase mutants of Saccharomyces cerevisiae. J Bacteriol 156(1):36-48
2) Enenkel C and Wolf DH  (1993) BLH1 codes for a yeast thiol aminopeptidase, the equivalent of mammalian bleomycin hydrolase. J Biol Chem 268(10):7036-43
3) Zheng W, et al.  (1997) The cysteine-peptidase bleomycin hydrolase is a member of the galactose regulon in yeast. J Biol Chem 272(48):30350-5
4) Kambouris NG, et al.  (1992) Cloning and characterization of a cysteine proteinase from Saccharomyces cerevisiae. J Biol Chem 267(30):21570-6
5) Xu HE and Johnston SA  (1994) Yeast bleomycin hydrolase is a DNA-binding cysteine protease. Identification, purification, biochemical characterization. J Biol Chem 269(33):21177-83
6) Zimny J, et al.  (2006) Protective mechanisms against homocysteine toxicity: the role of bleomycin hydrolase. J Biol Chem 281(32):22485-92
7) Ferrando AA, et al.  (1996) Cloning and expression analysis of human bleomycin hydrolase, a cysteine proteinase involved in chemotherapy resistance. Cancer Res 56(8):1746-50