SQS1/YNL224C Summary

SQS1 BASIC INFORMATION

Standard Name	SQS1 1
Systematic Name	YNL224C
Alias	PFA1 2
Feature Type	ORF, Verified
Description	Stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles (1, 2, 3, 4)
Name Description	SQuelch of Splicing suppression 1

GO Annotations	All SQS1 GO evidence and references
	View Computational GO annotations for SQS1
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	maturation of SSU-rRNA (IGI) nuclear mRNA splicing, via spliceosome (IGI) positive regulation of ATPase activity (IDA, IMP) positive regulation of helicase activity (IDA, IMP) rRNA processing (IGI)
Cellular Component
Manually curated	90S preribosome (IDA) preribosome, large subunit precursor (IDA) preribosome, small subunit precursor (IDA)
High-throughput	cytoplasm (IDA) nucleus (IDA)

Mutant Phenotype	All SQS1 Phenotype details and references
Classical genetics
null	viable
Large-scale survey
null	hyperosmotic stress resistance: decreased resistance to cycloheximide: decreased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to ethanol: decreased transposable element transposition: decreased viable

Interactions	SQS1 All interactions details and references
	57 total interaction(s) for 47 unique genes/features.
Physical Interactions	Affinity Capture-MS: 12 Affinity Capture-RNA: 1 Biochemical Activity: 1 Two-hybrid: 2
Genetic Interactions	Dosage Lethality: 2 Phenotypic Enhancement: 23 Phenotypic Suppression: 5 Synthetic Growth Defect: 9 Synthetic Lethality: 1 Synthetic Rescue: 1

Sequence Information

ChrXIV:227100 to 224797 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2005-11-07 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2304	227100..224797	2005-11-07	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000005168

SQS1 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for SQS1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SQS1]

1)	Pandit S, et al. (2006) Inhibition of a spliceosome turnover pathway suppresses splicing defects. Proc Natl Acad Sci U S A 103(37):13700-5
2)	Lebaron S, et al. (2005) The splicing ATPase prp43p is a component of multiple preribosomal particles. Mol Cell Biol 25(21):9269-82
3)	Pertschy B, et al. (2009) RNA helicase Prp43 and its co-factor Pfa1 promote 20 to 18 S rRNA processing catalyzed by the endonuclease Nob1. J Biol Chem 284(50):35079-91
4)	Lebaron S, et al. (2009) The ATPase and helicase activities of Prp43p are stimulated by the G-patch protein Pfa1p during yeast ribosome biogenesis. EMBO J 28(24):3808-19

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