SQS1/YNL224C Summary

SQS1/YNL224C Summary

Standard Name	SQS1 1
Systematic Name	YNL224C
Alias	PFA1 2
Feature Type	ORF, Verified
Description	Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress (1, 2, 3, 4, 5)
Name Description	SQuelch of Splicing suppression 1

Chromosomal Location	ChrXIV:227099 to 224796 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All SQS1 GO evidence and references

	View Computational GO annotations for SQS1
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	maturation of SSU-rRNA (IGI) mRNA splicing, via spliceosome (IGI) positive regulation of ATPase activity (IDA, IMP) positive regulation of helicase activity (IDA, IMP) rRNA processing (IGI)
Cellular Component
Manually curated	90S preribosome (IDA) preribosome, large subunit precursor (IDA) preribosome, small subunit precursor (IDA)
High-throughput	cytoplasm (IDA) nucleolus (IDA) nucleus (IDA)

Mutant phenotypes All SQS1 Phenotype evidence and references

Classical genetics
null	heat sensitivity: increased resistance to benomyl: decreased viable
overexpression	cell cycle progression in M phase: decreased
Large-scale survey
null	competitive fitness: decreased dessication resistance: decreased hyperosmotic stress resistance: decreased resistance to camptothecin: increased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to ethanol: decreased resistance to cycloheximide: decreased resistance to hydroxyurea: decreased resistance to methyl methanesulfonate: increased resistance to paromomycin: decreased stress resistance: decreased transposable element transposition: decreased viable
overexpression	cell cycle progression: abnormal vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All SQS1 Interaction evidence and references

	78 total interaction(s) for 65 unique genes/features.
Physical Interactions	Affinity Capture-MS: 12 Affinity Capture-RNA: 5 Affinity Capture-Western: 2 Biochemical Activity: 1 Two-hybrid: 2
Genetic Interactions	Dosage Lethality: 2 Negative Genetic: 26 Phenotypic Enhancement: 1 Positive Genetic: 12 Synthetic Growth Defect: 13 Synthetic Lethality: 1 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All SQS1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXIV:227099 to 224796 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2304	227099..224796	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005168

References cited on this page View Complete Literature Guide for SQS1

1)	Pandit S, et al. (2006) Inhibition of a spliceosome turnover pathway suppresses splicing defects. Proc Natl Acad Sci U S A 103(37):13700-5
2)	Lebaron S, et al. (2005) The splicing ATPase prp43p is a component of multiple preribosomal particles. Mol Cell Biol 25(21):9269-82
3)	Pertschy B, et al. (2009) RNA helicase Prp43 and its co-factor Pfa1 promote 20 to 18 S rRNA processing catalyzed by the endonuclease Nob1. J Biol Chem 284(50):35079-91
4)	Lebaron S, et al. (2009) The ATPase and helicase activities of Prp43p are stimulated by the G-patch protein Pfa1p during yeast ribosome biogenesis. EMBO J 28(24):3808-19
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76