UBP10/YNL186W Summary Help

Standard Name UBP10 1
Systematic Name YNL186W
Alias DOT4 2
Feature Type ORF, Verified
Description Ubiquitin-specific protease, deubiquitinates Ub-protein moieties; interacts with proteins that function in rRNA production and ribosome biogenesis; stabilizes Rpa190p, the largest subunit of RNAP I, by mediating its deubiquitination; controls PCNA deubiquitylation; may regulate silencing by acting on Sir4p; involved in posttranscriptionally regulating Gap1p and possibly other transporters; localized to the nucleolus; human USP36 is a functional analog of Ubp10p (2, 3, 4, 5, 6)
Name Description UBiquitin-specific Protease 1
Chromosomal Location
ChrXIV:289498 to 291876 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All UBP10 GO evidence and references
  View Computational GO annotations for UBP10
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 6 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
overexpression
Resources
182 total interaction(s) for 153 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 101
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 6
  • Biochemical Activity: 29
  • PCA: 14
  • Protein-peptide: 4
  • Reconstituted Complex: 3
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 1
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 2
  • Synthetic Growth Defect: 4
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 792
Molecular Weight (Da) 88,531
Isoelectric Point (pI) 5.17
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:289498 to 291876 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2379 289498..291876 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005130
References cited on this page View Complete Literature Guide for UBP10
1) Amerik AY, et al.  (2000) Analysis of the deubiquitinating enzymes of the yeast Saccharomyces cerevisiae. Biol Chem 381(9-10):981-92
2) Singer MS, et al.  (1998) Identification of high-copy disruptors of telomeric silencing in Saccharomyces cerevisiae. Genetics 150(2):613-32
3) Kahana A and Gottschling DE  (1999) DOT4 links silencing and cell growth in Saccharomyces cerevisiae. Mol Cell Biol 19(10):6608-20
4) Kahana A  (2001) The deubiquitinating enzyme Dot4p is involved in regulating nutrient uptake. Biochem Biophys Res Commun 282(4):916-20
5) Gallego-Sanchez A, et al.  (2012) Reversal of PCNA Ubiquitylation by Ubp10 in Saccharomyces cerevisiae. PLoS Genet 8(7):e1002826
6) Richardson LA, et al.  (2012) A conserved deubiquitinating enzyme controls cell growth by regulating RNA polymerase I stability. Cell Rep 2(2):372-85