THO2/YNL139C Summary

THO2/YNL139C Summary

Standard Name	THO2 1
Systematic Name	YNL139C
Alias	ZRG13 2 , LDB5 , RLR1 3
Feature Type	ORF, Verified
Description	Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids (1, 3, 4, 5)
Name Description	suppressor of the Transcriptional defect of Hpr1 by Overexpression 1

Chromosomal Location	ChrXIV:365717 to 360924 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All THO2 GO evidence and references

	View Computational GO annotations for THO2
Molecular Function
Manually curated	nucleic acid binding (IDA)
High-throughput	DNA binding (IPI)
Biological Process
Manually curated	DNA recombination (IMP) mRNA 3'-end processing (IMP) mRNA export from nucleus (IMP) transcription elongation from RNA polymerase II promoter (IMP) transcription-coupled nucleotide-excision repair (IMP)
Cellular Component
Manually curated	nucleoplasmic THO complex (IMP, IPI) nucleus (IDA) THO complex part of transcription export complex (IPI)
High-throughput	nucleus (IDA)

Regulatory Role


Resources	ScerTF \| YeTFaSCo

Mutant phenotypes All THO2 Phenotype evidence and references

Classical genetics
null	mRNAs accumulation: decreased growth in exponential phase: decreased rate heat sensitivity: increased mitotic recombination: decreased mitotic recombination: increased vegetative growth: decreased viable
Large-scale survey
null	HSP104 RNA accumulation: abnormal UV resistance: decreased budding pattern: abnormal cell shape: abnormal cell size: increased polyphosphate accumulation: abnormal competitive fitness: decreased dessication resistance: decreased fermentative growth: decreased rate haploproficient heat sensitivity: increased resistance to ethanol: decreased resistance to camptothecin: decreased resistance to hydroxyurea: decreased resistance to quinine: increased resistance to sulfanilamide: decreased resistance to CTBT: decreased resistance to amiodarone: decreased resistance to lovastatin: decreased resistance to calcium dichloride: decreased resistance to caffeine: decreased resistance to cycloheximide: decreased resistance to hygromycin B: decreased resistance to 5-fluorouracil: decreased resistance to sorbate: decreased resistance to doxorubicin: decreased resistance to sirolimus: decreased respiratory growth: decreased rate utilization of phosphorus source: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All THO2 Interaction evidence and references

	115 total interaction(s) for 46 unique genes/features.
Physical Interactions	Affinity Capture-MS: 55 Affinity Capture-RNA: 2 Affinity Capture-Western: 17 Co-crystal Structure: 4 Co-purification: 4 Two-hybrid: 1
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 3 Negative Genetic: 3 Phenotypic Enhancement: 5 Phenotypic Suppression: 2 Synthetic Growth Defect: 2 Synthetic Lethality: 12 Synthetic Rescue: 4
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All THO2 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXIV:365717 to 360924 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..4794	365717..360924	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005083

References cited on this page View Complete Literature Guide for THO2

1)	Piruat JI and Aguilera A (1998) A novel yeast gene, THO2, is involved in RNA pol II transcription and provides new evidence for transcriptional elongation-associated recombination. EMBO J 17(16):4859-72
2)	Yuan DS (2000) Zinc-regulated genes in Saccharomyces cerevisiae revealed by transposon tagging. Genetics 156(1):45-58
3)	West RW Jr, et al. (2000) RLR1 (THO2), required for expressing lacZ fusions in yeast, is conserved from yeast to humans and is a suppressor of SIN4. Gene 243(1-2):195-205
4)	Chavez S, et al. (2000) A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae. EMBO J 19(21):5824-34
5)	Rondon AG, et al. (2003) Molecular evidence that the eukaryotic THO/TREX complex is required for efficient transcription elongation. J Biol Chem 278(40):39037-43