NAF1/YNL124W Summary Help

Standard Name NAF1 1
Systematic Name YNL124W
Feature Type ORF, Verified
Description RNA-binding protein required for the assembly of box H/ACA snoRNPs; thus required for pre-rRNA processing; forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p (1, 2, 3)
Name Description Nuclear Assembly Factor 1
Chromosomal Location
ChrXIV:392892 to 394370 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All NAF1 GO evidence and references
  View Computational GO annotations for NAF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
null
repressible
Large-scale survey
null
reduction of function
unspecified
Resources
116 total interaction(s) for 95 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 91
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 7
  • Reconstituted Complex: 4
  • Two-hybrid: 11

Genetic Interactions
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 492
Molecular Weight (Da) 54,949
Isoelectric Point (pI) 4.51
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:392892 to 394370 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1479 392892..394370 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005068
References cited on this page View Complete Literature Guide for NAF1
1) Fatica A, et al.  (2002) Naf1 p is a box H/ACA snoRNP assembly factor. RNA 8(12):1502-14
2) Dez C, et al.  (2002) Naf1p, an essential nucleoplasmic factor specifically required for accumulation of box H/ACA small nucleolar RNPs. Mol Cell Biol 22(20):7053-65
3) Yang PK, et al.  (2002) The Shq1p.Naf1p complex is required for box H/ACA small nucleolar ribonucleoprotein particle biogenesis. J Biol Chem 277(47):45235-42