DCP2/YNL118C Summary Help

Standard Name DCP2 1
Systematic Name YNL118C
Alias PSU1
Feature Type ORF, Verified
Description Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; also enters the nucleus and positively regulates transcription initiation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress (2, 3, 4, 5, 6, 7)
Name Description mRNA DeCaPping 1
Chromosomal Location
ChrXIV:405564 to 402652 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All DCP2 GO evidence and references
  View Computational GO annotations for DCP2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 6 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
conditional
null
Resources
221 total interaction(s) for 153 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 51
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 9
  • Biochemical Activity: 2
  • Co-fractionation: 1
  • Co-purification: 1
  • PCA: 12
  • Reconstituted Complex: 9
  • Two-hybrid: 44

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 67
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 2
  • Positive Genetic: 7
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 970
Molecular Weight (Da) 108,666
Isoelectric Point (pI) 5.72
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:405564 to 402652 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2913 405564..402652 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005062
References cited on this page View Complete Literature Guide for DCP2
1) Dunckley T and Parker R  (1999) The DCP2 protein is required for mRNA decapping in Saccharomyces cerevisiae and contains a functional MutT motif. EMBO J 18(19):5411-22
2) Gaudon C, et al.  (1999) Role of the essential yeast protein PSU1 in p6anscriptional enhancement by the ligand-dependent activation function AF-2 of nuclear receptors. EMBO J 18(8):2229-40
3) Dunckley T, et al.  (2001) Two related proteins, Edc1p and Edc2p, stimulate mRNA decapping in Saccharomyces cerevisiae. Genetics 157(1):27-37
4) She M, et al.  (2006) Crystal structure and functional analysis of Dcp2p from Schizosaccharomyces pombe. Nat Struct Mol Biol 13(1):63-70
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6) Song MG, et al.  (2013) Multiple Nudix family proteins possess mRNA decapping activity. RNA 19(3):390-9
7) Haimovich G, et al.  (2013) Gene expression is circular: factors for mRNA degradation also foster mRNA synthesis. Cell 153(5):1000-11