Standard Name	TCB2 1
Systematic Name	YNL087W
Feature Type	ORF, Verified
Description	ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to bud; TCB2 has a paralog, TCB1, that arose from the whole genome duplication (2, 3, 4, 5)
Name Description	Three Calcium and lipid Binding domains (TriCalBins) 1

Chromosomal Location	ChrXIV:462411 to 465947 | ORF Map | GBrowse

	View Computational GO annotations for TCB2
Molecular Function
Manually curated	lipid binding (IDA)
Biological Process
Manually curated	endoplasmic reticulum membrane organization (IGI) regulation of phosphatidylinositol dephosphorylation (IGI)
Cellular Component
Manually curated	cellular bud (IDA) cortical endoplasmic reticulum (IDA)

Classical genetics
null	resistance to myriocin: decreased resistance to cycloheximide: decreased vacuolar morphology: abnormal viable
Large-scale survey
null	competitive fitness: decreased protein transport: abnormal resistance to arsenite(3-): decreased resistance to neomycin: decreased resistance to cycloheximide: decreased resistance to bleomycin: increased resistance to tunicamycin: decreased resistance to hydroxyurea: increased resistance to cycloheximide: increased resistance to camptothecin: increased viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	41 total interaction(s) for 39 unique genes/features.
Physical Interactions	Affinity Capture-MS: 13 Affinity Capture-RNA: 2 PCA: 1 Two-hybrid: 3
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 15 Phenotypic Enhancement: 1 Synthetic Growth Defect: 3 Synthetic Lethality: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXIV:462411 to 465947 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..3537 462411..465947 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005031