TCB2/YNL087W Summary Help

Standard Name TCB2 1
Systematic Name YNL087W
Feature Type ORF, Verified
Description ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to bud; TCB2 has a paralog, TCB1, that arose from the whole genome duplication (2, 3, 4, 5)
Name Description Three Calcium and lipid Binding domains (TriCalBins) 1
Chromosomal Location
ChrXIV:462411 to 465947 | ORF Map | GBrowse
Gene Ontology Annotations All TCB2 GO evidence and references
  View Computational GO annotations for TCB2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 9 genes
Classical genetics
Large-scale survey
44 total interaction(s) for 40 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 16
  • Affinity Capture-RNA: 2
  • PCA: 1
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 15
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 2

Expression Summary
Length (a.a.) 1,178
Molecular Weight (Da) 132,508
Isoelectric Point (pI) 9.04
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIV:462411 to 465947 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3537 462411..465947 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005031
References cited on this page View Complete Literature Guide for TCB2
1) Creutz CE, et al.  (2004) Characterization of the yeast tricalbins: membrane-bound multi-C2-domain proteins that form complexes involved in membrane trafficking. Cell Mol Life Sci 61(10):1208-20
2) Shepard KA, et al.  (2003) Widespread cytoplasmic mRNA transport in yeast: identification of 22 bud-localized transcripts using DNA microarray analysis. Proc Natl Acad Sci U S A 100(20):11429-34
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Toulmay A and Prinz WA  (2012) A conserved membrane-binding domain targets proteins to organelle contact sites. J Cell Sci 125(Pt 1):49-58
5) Manford AG, et al.  (2012) ER-to-Plasma Membrane Tethering Proteins Regulate Cell Signaling and ER Morphology. Dev Cell 23(6):1129-40