NOP2/YNL061W Summary Help

Standard Name NOP2 1
Systematic Name YNL061W
Alias YNA1
Feature Type ORF, Verified
Description rRNA m5C methyltransferase; methylates cytosine at position 2870 of 25S rRNA while Rcm1p methylates cytosine at position 2278; contains seven beta-strand methyltransferase motif; essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles; homolog of p120/NSUN1, a human gene upregulated in cancer (1, 2, 3, 4, 5, 6)
Name Description NucleOlar Protein 1
Chromosomal Location
ChrXIV:510540 to 512396 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All NOP2 GO evidence and references
  View Computational GO annotations for NOP2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
unspecified
Large-scale survey
null
overexpression
reduction of function
repressible
Resources
254 total interaction(s) for 136 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 206
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 5
  • Two-hybrid: 6

Genetic Interactions
  • Negative Genetic: 22
  • Positive Genetic: 1
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 618
Molecular Weight (Da) 69,812
Isoelectric Point (pI) 4.79
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:510540 to 512396 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1857 510540..512396 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005005
References cited on this page View Complete Literature Guide for NOP2
1) de Beus E, et al.  (1994) Yeast NOP2 encodes an essential nucleolar protein with homology to a human proliferation marker. J Cell Biol 127(6 Pt 2):1799-813
2) King M, et al.  (1999) A conserved motif in the yeast nucleolar protein Nop2p contains an essential cysteine residue. Biochem J 337 ( Pt 1)():29-35
3) Hong B, et al.  (2001) Temperature sensitive nop2 alleles defective in synthesis of 25S rRNA and large ribosomal subunits in Saccharomyces cerevisiae. Nucleic Acids Res 29(14):2927-37
4) Horsey EW, et al.  (2004) Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation. RNA 10(5):813-27
5) Petrossian TC and Clarke SG  (2009) Multiple Motif Scanning to identify methyltransferases from the yeast proteome. Mol Cell Proteomics 8(7):1516-26
6) Sharma S, et al.  (2013) Yeast Nop2 and Rcm1 methylate C2870 and C2278 of the 25S rRNA, respectively. Nucleic Acids Res 41(19):9062-76