Standard Name	ASI3
Systematic Name	YNL008C
Feature Type	ORF, Verified
Description	Putative integral membrane E3 ubiquitin ligase; acts with Asi1p and Asi2p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI3 has a paralog, ASI1, that arose from the whole genome duplication (1, 2, 3)
Name Description	Amino acid Sensor-Independent 1

Chromosomal Location	ChrXIV:618241 to 616211 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for ASI3
Molecular Function
Manually curated	ubiquitin-protein ligase activity (IGI, ISS)
Biological Process
Manually curated	ubiquitin-dependent protein catabolic process (IGI)
Cellular Component
Manually curated	endoplasmic reticulum membrane (IGI, ISS) nuclear inner membrane (IDA)
High-throughput	integral to membrane (ISM)

Large-scale survey
null	competitive fitness: increased resistance to selenomethionine: increased resistance to tirapazamine: increased starvation resistance: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	37 total interaction(s) for 29 unique genes/features.
Physical Interactions	Affinity Capture-MS: 3 Affinity Capture-RNA: 1 Affinity Capture-Western: 1 PCA: 3
Genetic Interactions	Negative Genetic: 23 Phenotypic Enhancement: 1 Positive Genetic: 2 Synthetic Rescue: 3
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXIV:618241 to 616211 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2031 618241..616211 2011-02-03 2011-02-03
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000004953