CAT8/YMR280C Summary Help

Standard Name CAT8 1
Systematic Name YMR280C
Alias DIL1 2 , MSP8
Feature Type ORF, Verified
Description Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress (1, 3, 4, 5, 6, 7, 8, 9 and see Summary Paragraph)
Name Description CATabolite repression 10
Chromosomal Location
ChrXIII:831329 to 827028 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All CAT8 GO evidence and references
  View Computational GO annotations for CAT8
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Targets 227 genes
Regulators 137 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
37 total interaction(s) for 26 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 1
  • Co-localization: 1
  • Protein-peptide: 1
  • Two-hybrid: 9

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 8
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 2
  • Positive Genetic: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,433
Molecular Weight (Da) 160,484
Isoelectric Point (pI) 9.73
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:831329 to 827028 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4302 831329..827028 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004893
SUMMARY PARAGRAPH for CAT8

CAT8 encodes a zinc-finger cluster protein that mediates derepression of a number of genes during the diauxic shift, which is the transition between fermentative and nonfermentative metabolism (1). Genomic studies have shown that least 30 genes, encoding proteins involved in gluconeogenesis, ethanol utilization, and the glyoxylate cycle, are regulated by Cat8p (3, 11). In addition, Cat8p and the transcription factor Adr1p coregulate a number of genes (11).

Under conditions in which glucose is plentiful, expression of CAT8 is repressed by the DNA binding protein Mig1p, which recruits the repressor complex Ssn6p-Tup1p and binds to a site in the CAT8 promoter (1, 12). When availability of glucose decreases, Mig1p is phosphorylated and transported to the cytoplasm, relieving repression of Cat8p and likely recruiting a transcription activator of CAT8 expression as well (13). Cat8p functions to derepress transcription of target genes by binding to the CSRE (carbon source-responsive element) upstream of these genes (12, 14). At least some of the genes activated by Cat8p encode additional transcription factors such as Sip4p, which functions in later steps of the derepression process (14).

While glucose regulates transcription of CAT8, it also appears to regulate Cat8p activity; Cat8p is phosphorylated in derepressed cells and addition of glucose triggers dephosphorylation (12).

Last updated: 2005-08-23 Contact SGD

References cited on this page View Complete Literature Guide for CAT8
1) Hedges D, et al.  (1995) CAT8, a new zinc cluster-encoding gene necessary for derepression of gluconeogenic enzymes in the yeast Saccharomyces cerevisiae. Mol Cell Biol 15(4):1915-22
2) Rahner A, et al.  (1996) Dual influence of the yeast Cat1p (Snf1p) protein kinase on carbon source-dependent transcriptional activation of gluconeogenic genes by the regulatory gene CAT8. Nucleic Acids Res 24(12):2331-7
3) Haurie V, et al.  (2001) The transcriptional activator Cat8p provides a major contribution to the reprogramming of carbon metabolism during the diauxic shift in Saccharomyces cerevisiae. J Biol Chem 276(1):76-85
4) Bojunga N and Entian KD  (1999) Cat8p, the activator of gluconeogenic genes in Saccharomyces cerevisiae, regulates carbon source-dependent expression of NADP-dependent cytosolic isocitrate dehydrogenase (Idp2p) and lactate permease (Jen1p). Mol Gen Genet 262(4-5):869-75
5) Vincent O and Carlson M  (1998) Sip4, a Snf1 kinase-dependent transcriptional activator, binds to the carbon source-responsive element of gluconeogenic genes. EMBO J 17(23):7002-8
6) Bojunga N, et al.  (1998) The succinate/fumarate transporter Acr1p of Saccharomyces cerevisiae is part of the gluconeogenic pathway and its expression is regulated by Cat8p. Mol Gen Genet 260(5):453-61
7) Hiesinger M, et al.  (2001) Contribution of Cat8 and Sip4 to the transcriptional activation of yeast gluconeogenic genes by carbon source-responsive elements. Curr Genet 39(2):68-76
8) Young ET, et al.  (2003) Multiple pathways are co-regulated by the protein kinase Snf1 and the transcription factors Adr1 and Cat8. J Biol Chem 278(28):26146-58
9) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
10) Zimmermann FK, et al.  (1977) Genetics of carbon catabolite repression in Saccharomycess cerevisiae: genes involved in the derepression process. Mol Gen Genet 151(1):95-103
11) Tachibana C, et al.  (2005) Combined global localization analysis and transcriptome data identify genes that are directly coregulated by Adr1 and Cat8. Mol Cell Biol 25(6):2138-46
12) Randez-Gil F, et al.  (1997) Glucose derepression of gluconeogenic enzymes in Saccharomyces cerevisiae correlates with phosphorylation of the gene activator Cat8p. Mol Cell Biol 17(5):2502-10
13) De Vit MJ, et al.  (1997) Regulated nuclear translocation of the Mig1 glucose repressor. Mol Biol Cell 8(8):1603-18
14) Roth S, et al.  (2004) Transcriptional activators Cat8 and Sip4 discriminate between sequence variants of the carbon source-responsive promoter element in the yeast Saccharomyces cerevisiae. Curr Genet 45(3):121-8
15) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87