PEP5/YMR231W Summary Help

Standard Name PEP5 1
Systematic Name YMR231W
Alias END1 2 , VAM1 , VPL9 3 , VPS11 3 , 4 , VPT11 4
Feature Type ORF, Verified
Description Histone E3 ligase, component of CORVET membrane tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p; involved in ubiquitylation and degradation of excess histones (5, 6, 7, 8, 9)
Name Description carboxyPEPtidase Y-deficient 10
Chromosomal Location
ChrXIII:733545 to 736634 | ORF Map | GBrowse
Genetic position: 152 cM
Gene Ontology Annotations All PEP5 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
reduction of function
Large-scale survey
160 total interaction(s) for 69 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 23
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 40
  • Biochemical Activity: 1
  • Co-purification: 2
  • PCA: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 29

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 2
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 6
  • Synthetic Growth Defect: 23
  • Synthetic Lethality: 16
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 1,029
Molecular Weight (Da) 117,476
Isoelectric Point (pI) 5.67
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:733545 to 736634 | ORF Map | GBrowse
Genetic position: 152 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3090 733545..736634 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004844
References cited on this page View Complete Literature Guide for PEP5
1) Woolford, C., et al.  (1989) Personal Communication, Mortimer Map Edition 10
2) Chvatchko Y, et al.  (1986) Two yeast mutants defective in endocytosis are defective in pheromone response. Cell 46(3):355-64
3) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
4) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
5) Srivastava A, et al.  (2000) Pep3p/Pep5p complex: a putative docking factor at multiple steps of vesicular transport to the vacuole of Saccharomyces cerevisiae. Genetics 156(1):105-22
6) Dulic V and Riezman H  (1989) Characterization of the END1 gene required for vacuole biogenesis and gluconeogenic growth of budding yeast. EMBO J 8(5):1349-59
7) Peplowska K, et al.  (2007) The CORVET Tethering Complex Interacts with the Yeast Rab5 Homolog Vps21 and Is Involved in Endo-Lysosomal Biogenesis. Dev Cell 12(5):739-50
8) Singh RK, et al.  (2012) Novel E3 Ubiquitin Ligases That Regulate Histone Protein Levels in the Budding Yeast Saccharomyces cerevisiae. PLoS One 7(5):e36295
9) Balderhaar HJ, et al.  (2013) The CORVET complex promotes tethering and fusion of Rab5/Vps21-positive membranes. Proc Natl Acad Sci U S A 110(10):3823-8
10) Jones EW  (1977) Proteinase mutants of Saccharomyces cerevisiae. Genetics 85(1):23-33