SGS1/YMR190C Summary Help

SGS1 BASIC INFORMATION

Standard Name SGS1 1
Systematic Name YMR190C
Feature Type ORF, Verified
Description Nucleolar DNA helicase of the RecQ family involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes (2, 3, 4, 5, 6, 7 and see Summary Paragraph)
Name Description Slow Growth Suppressor
GO Annotations All SGS1 GO evidence and references
    View Computational GO annotations for SGS1
Molecular Function
Manually curated
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
Mutant Phenotype All SGS1 Phenotype details and references
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Interactions SGS1 All interactions details and references
550 total interaction(s) for 172 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-Western: 18
  • Biochemical Activity: 4
  • Co-fractionation: 3
  • Co-localization: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 20
Genetic Interactions
  • Dosage Lethality: 3
  • Dosage Rescue: 6
  • Phenotypic Enhancement: 135
  • Phenotypic Suppression: 25
  • Synthetic Growth Defect: 158
  • Synthetic Lethality: 136
  • Synthetic Rescue: 36
Sequence Information
ChrXIII:645257 to 640914 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 115.6 cM
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..4344 645257..640914 1996-07-31 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000004802

SGS1 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Localization Resources
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for SGS1

SUMMARY PARAGRAPH for SGS1

SGS1 encodes a helicase with similarity to E. coli RecQ and human BLM and WRN helicases (8). Mutations in BLM are implicated in the cancer-prone Bloom's Syndrome, and mutations in WRN cause the premature-aging Werner's Syndrome (8). SGS1 was identified in a screen for suppressors of the slow growth phenotype of top3 mutants, and Sgs1p has been shown to interact with the topoisomerase Top3p (9). Sgs1p appears to be involved in the maintenance of genome stability and the suppression of illegitimate recombination; sgs1 null mutants show mitotic hyperrecombination and elevated levels of chromosome missegregation (10, 11, 12). Sgs1p has been localized to the nucleolus, and is needed to maintain the integrity of rDNA repeats (2). Sgs1p shows ATPase activity and unwinds duplex DNA; it preferentially binds to branched DNA substrates and has a 3' to 5' polarity of unwinding (13, 14).

Last updated: 2000-03-10

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SGS1]

1) Sternglanz, R.  (1993) Personal Communication, Mortimer Map Edition 12
2) Sun H, et al.  (1999) The Saccharomyces cerevisiae Sgs1 helicase efficiently unwinds G-G paired DNAs. Nucleic Acids Res 27(9):1978-84
3) Huber MD, et al.  (2002) G4 DNA unwinding by BLM and Sgs1p: substrate specificity and substrate-specific inhibition. Nucleic Acids Res 30(18):3954-61
4) Versini G, et al.  (2003) The yeast Sgs1 helicase is differentially required for genomic and ribosomal DNA replication. EMBO J 22(8):1939-49
5) Rockmill B, et al.  (2003) The Sgs1 helicase regulates chromosome synapsis and meiotic crossing over. Curr Biol 13(22):1954-62
6) Oh SD, et al.  (2008) RecQ helicase, Sgs1, and XPF family endonuclease, Mus81-Mms4, resolve aberrant joint molecules during meiotic recombination. Mol Cell 31(3):324-36
7) Jessop L and Lichten M  (2008) Mus81/Mms4 endonuclease and Sgs1 helicase collaborate to ensure proper recombination intermediate metabolism during meiosis. Mol Cell 31(3):313-23
8) Kusano K, et al.  (1999) Evolution of the RECQ family of helicases: A drosophila homolog, Dmblm, is similar to the human bloom syndrome gene. Genetics 151(3):1027-39
9) Gangloff S, et al.  (1994) The yeast type I topoisomerase Top3 interacts with Sgs1, a DNA helicase homolog: a potential eukaryotic reverse gyrase. Mol Cell Biol 14(12):8391-8
10) Watt PM, et al.  (1995) Sgs1: a eukaryotic homolog of E. coli RecQ that interacts with topoisomerase II in vivo and is required for faithful chromosome segregation. Cell 81(2):253-60
11) Watt PM, et al.  (1996) SGS1, a homologue of the Bloom's and Werner's syndrome genes, is required for maintenance of genome stability in Saccharomyces cerevisiae. Genetics 144(3):935-45
12) Yamagata K, et al.  (1998) Bloom's and Werner's syndrome genes suppress hyperrecombination in yeast sgs1 mutant: implication for genomic instability in human diseases. Proc Natl Acad Sci U S A 95(15):8733-8
13) Bennett RJ, et al.  (1998) Purification and characterization of the Sgs1 DNA helicase activity of Saccharomyces cerevisiae. J Biol Chem 273(16):9644-50
14) Bennett RJ, et al.  (1999) Binding specificity determines polarity of DNA unwinding by the Sgs1 protein of S. cerevisiae. J Mol Biol 289(2):235-48