GCV2/YMR189W Summary Help

GCV2 BASIC INFORMATION

Standard Name GCV2 1
Systematic Name YMR189W
Alias GSD2
Feature Type ORF, Verified
Description P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm (1, 2, 3 and see Summary Paragraph)
Name Description GlyCine cleaVage 1
GO Annotations All GCV2 GO evidence and references
    View Computational GO annotations for GCV2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Pathways
Mutant Phenotype All GCV2 Phenotype details and references
Large-scale survey
null
Sequence Information
ChrXIII:637499 to 640603 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..3105 637499..640603 1996-07-31 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000004801

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Expression Summary histogram

ADDITIONAL INFORMATION for GCV2

SUMMARY PARAGRAPH for GCV2

GCV2 encodes a glycine decarboxylase known as the P subunit of the glycine cleavage system or glycine decarboxylase complex (GDC). GDC is a multienzyme complex that catalyzes the reversible oxidative cleavage of glycine into CO2 and NH3 and connects the metabolism of one, two and three-carbon compounds as shown in this pathway diagram. The other subunits of the GDC complex are: Gcv1p (the T-protein), a tetrahydrofolate transferase, Gcv3p (the H-protein), a carrier protein containing lipoamide, and Lpd1p (the L-protein), a lipoamide dehydrogenase. Gcv1p, Gcv2p and Gcv3p are unique to the GDC complex but Lpd1p acts in several other complexes including pyruvate dehydrogenase, 2-oxoglutarate dehydrogenase, and a branched chain oxo-acid dehydrogenase (4, 5, 1). GCV1, GCV2 and GCV3 are collectively referred to as the GCV genes and are up-regulated by glycine and one-carbon unit levels (5,10-CH2-H4folate).

GCV2 expression is induced by glycine and methionine and repressed in rich medium or by nitrogen sources (1, 6). GCV2 transcription is regulated, in different nutritional conditions, by three proteins: a Glycine Regulatory Region (GRR)-binding protein that binds to an 11bp sequence (TGACTCTTCTT), a GATA-binding protein of the nitrogen catabolite repression (NCR) set of transcription factors and a protein that binds a palindrome sequence (AAGGaCCTT) (7). Although the GRR region contains a consensus binding site for Bas1p transcription factor, conflicting reports have been published on the role of Bas1p in regulating GCV2 expression in response to glycine (8, 3, 9). While mutation of the Bas1p binding site doesn't affect glycine response (8), loss of Bas1p reduces the repression of GCV2 in response to glycine withdrawal (9).

Gcv2p is highly conserved from bacteria to humans (1). Mutations in the human glycine decarboxylase gene (GLDC) result in Glycine encephalopathy or Nonketotic hyperglycinemia (NKH) (10).

Last updated: 2007-12-11

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for GCV2]

1) Sinclair DA, et al.  (1996) Specific induction by glycine of the gene for the P-subunit of glycine decarboxylase from Saccharomyces cerevisiae. Mol Microbiol 19(3):611-23
2) Piper MD, et al.  (2000) Regulation of the balance of one-carbon metabolism in Saccharomyces cerevisiae. J Biol Chem 275(40):30987-95
3) Gelling CL, et al.  (2004) Identification of a novel one-carbon metabolism regulon in Saccharomyces cerevisiae. J Biol Chem 279(8):7072-81
4) Ogur M, et al.  (1977) "Active" one-carbon generation in Saccharomyces cerevisiae. J Bacteriol 129(2):926-33
5) Sinclair DA and Dawes IW  (1995) Genetics of the synthesis of serine from glycine and the utilization of glycine as sole nitrogen source by Saccharomyces cerevisiae. Genetics 140(4):1213-22
6) Nagarajan L and Storms RK  (1997) Molecular characterization of GCV3, the Saccharomyces cerevisiae gene coding for the glycine cleavage system hydrogen carrier protein. J Biol Chem 272(7):4444-50
7) Piper MD, et al.  (2002) Regulation of the yeast glycine cleavage genes is responsive to the availability of multiple nutrients. FEMS Yeast Res 2(1):59-71
8) Hong SP, et al.  (1999) Control of expression of one-carbon metabolism genes of Saccharomyces cerevisiae is mediated by a tetrahydrofolate-responsive protein binding to a glycine regulatory region including a core 5'-CTTCTT-3' motif. J Biol Chem 274(15):10523-32
9) Subramanian M, et al.  (2005) Transcriptional regulation of the one-carbon metabolism regulon in Saccharomyces cerevisiae by Bas1p. Mol Microbiol 57(1):53-69
10) Spegel CF, et al.  (2007) Amperometric Response from the Glycolytic versus the Pentose Phosphate Pathway in Saccharomyces cerevisiae Cells. Anal Chem 79(23):8919-8926