NDI1/YML120C Summary Help

Standard Name NDI1 1
Systematic Name YML120C
Feature Type ORF, Verified
Description NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; involved in Mn and H2O2 induced apoptosis; upon apoptotic stress, Ndip is activated in the mitochondria by N-terminal cleavage, and the truncated protein translocates to the cytoplasm to induce apoptosis; homolog of human AMID (1, 2, 3, 4, 5)
Name Description NADH Dehydrogenase Internal 1
Chromosomal Location
ChrXIII:29807 to 28266 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NDI1 GO evidence and references
  View Computational GO annotations for NDI1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 7 genes
Resources
Pathways
Classical genetics
null
overexpression
Large-scale survey
null
Resources
43 total interaction(s) for 34 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-Western: 1
  • Co-crystal Structure: 2
  • Co-purification: 1
  • PCA: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Negative Genetic: 16
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 1
  • Positive Genetic: 5
  • Synthetic Lethality: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 513
Molecular Weight (Da) 57,249
Isoelectric Point (pI) 10.26
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:29807 to 28266 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1542 29807..28266 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004589
References cited on this page View Complete Literature Guide for NDI1
1) Li W, et al.  (2006) Yeast AMID homologue Ndi1p displays respiration-restricted apoptotic activity and is involved in chronological aging. Mol Biol Cell 17(4):1802-11
2) Marres CA, et al.  (1991) Isolation and inactivation of the nuclear gene encoding the rotenone-insensitive internal NADH: ubiquinone oxidoreductase of mitochondria from Saccharomyces cerevisiae. Eur J Biochem 195(3):857-62
3) Velazquez I and Pardo JP  (2001) Kinetic characterization of the rotenone-insensitive internal NADH: ubiquinone oxidoreductase of mitochondria from Saccharomyces cerevisiae. Arch Biochem Biophys 389(1):7-14
4) Reinders J, et al.  (2007) Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol Cell Proteomics 6(11):1896-906
5) Cui Y, et al.  (2012) Mitochondrial release of the NADH dehydrogenase Ndi1 induces apoptosis in yeast. Mol Biol Cell 23(22):4373-82