TSL1/YML100W Summary Help

Standard Name TSL1 1
Systematic Name YML100W
Feature Type ORF, Verified
Description Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; contributes to survival to acute lethal heat stress; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7 and see Summary Paragraph)
Name Description Trehalose Synthase Long chain 1
Chromosomal Location
ChrXIII:70624 to 73920 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All TSL1 GO evidence and references
  View Computational GO annotations for TSL1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 54 genes
Resources
Pathways
Classical genetics
null
overexpression
Large-scale survey
null
Resources
55 total interaction(s) for 34 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 27
  • Affinity Capture-RNA: 2
  • Biochemical Activity: 1
  • Two-hybrid: 4

Genetic Interactions
  • Negative Genetic: 9
  • Phenotypic Enhancement: 1
  • Positive Genetic: 8
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,098
Molecular Weight (Da) 123,017
Isoelectric Point (pI) 6.61
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:70624 to 73920 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3297 70624..73920 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004566
SUMMARY PARAGRAPH for TSL1

In Saccharomyces cerevisiae, trehalose is a major reserve carbohydrate involved in reponses to thermal, osmotic, oxidative, and ethanol stresses (8), as well as the suppression of denatured protein aggregation (9). Trehalose biosynthesis is a two-step process in which glucose 6-phosphate and UDP-glucose are converted by trehalose-6-phosphate synthase (TPS), encoded by TPS1, into alpha,alpha-trehalose 6-phosphate, which is then converted with water into trehalose and phosphate by trehalose-6-phosphate phosphatase (TPP), encoded by TPS2 (10). The trehalose biosynthetic pathway can affect glycolysis in that one of its intermediates, trehalose-6-phosphate, inhibits hexokinase activity, which restricts the influx of sugars to glycolysis during the switch to fermentative metabolism (11).

Tps1p and Tps2p are part of the alpha,alpha-trehalose-phosphate synthase complex (12, 13) with Tps3p and Tsl1p, regulatory proteins with partially overlapping functions (1), though some Tps1p appears to be present in the cell as a monomer (14). TPS1, TPS2, TPS3 and TSL1 are coinduced under stress conditions, and corepressed by the Ras-cAMP pathway (15).

Deletion of TPS1 results in loss of both TPS activity and trehalose biosynthesis (12), whereas deletion of TPS2 results in temperature sensitivity and loss of TPP activity (13). Deletion of either TPS1 or TPS2 results in a growth defect on fermentable carbon sources (12, 13). Deletion of either TPS3 or TSL1 has only mild effects, but deletion of both TPS3 and TSL1 results in significant reductions in TPS and TPP activities, as well as reduced trehalose biosynthesis (14).

Last updated: 2005-08-04 Contact SGD

References cited on this page View Complete Literature Guide for TSL1
1) Vuorio OE, et al.  (1993) Cloning of two related genes encoding the 56-kDa and 123-kDa subunits of trehalose synthase from the yeast Saccharomyces cerevisiae. Eur J Biochem 216(3):849-61
2) Ferreira JC, et al.  (1997) Trehalose accumulation in mutants of Saccharomyces cerevisiae deleted in the UDPG-dependent trehalose synthase-phosphatase complex. Biochim Biophys Acta 1335(1-2):40-50
3) Reinders A, et al.  (1997) Structural analysis of the subunits of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae and their function during heat shock. Mol Microbiol 24(4):687-95
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Torres EM, et al.  (2010) Identification of aneuploidy-tolerating mutations. Cell 143(1):71-83
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7) Levy SF, et al.  (2012) Bet hedging in yeast by heterogeneous, age-correlated expression of a stress protectant. PLoS Biol 10(5):e1001325
8) Pereira MD, et al.  (2001) Acquisition of tolerance against oxidative damage in Saccharomyces cerevisiae. BMC Microbiol 1():11
9) Singer MA and Lindquist S  (1998) Multiple effects of trehalose on protein folding in vitro and in vivo. Mol Cell 1(5):639-48
10) Francois J and Parrou JL  (2001) Reserve carbohydrates metabolism in the yeast Saccharomyces cerevisiae. FEMS Microbiol Rev 25(1):125-45
11) Hohmann S, et al.  (1996) Evidence for trehalose-6-phosphate-dependent and -independent mechanisms in the control of sugar influx into yeast glycolysis. Mol Microbiol 20(5):981-91
12) Bell W, et al.  (1992) Characterization of the 56-kDa subunit of yeast trehalose-6-phosphate synthase and cloning of its gene reveal its identity with the product of CIF1, a regulator of carbon catabolite inactivation. Eur J Biochem 209(3):951-9
13) De Virgilio C, et al.  (1993) Disruption of TPS2, the gene encoding the 100-kDa subunit of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae, causes accumulation of trehalose-6-phosphate and loss of trehalose-6-phosphate phosphatase activity. Eur J Biochem 212(2):315-23
14) Bell W, et al.  (1998) Composition and functional analysis of the Saccharomyces cerevisiae trehalose synthase complex. J Biol Chem 273(50):33311-9
15) Winderickx J, et al.  (1996) Regulation of genes encoding subunits of the trehalose synthase complex in Saccharomyces cerevisiae: novel variations of STRE-mediated transcription control? Mol Gen Genet 252(4):470-82