MFT1/YML062C Summary Help

Standard Name MFT1 1
Systematic Name YML062C
Alias MFT52
Feature Type ORF, Verified
Description Subunit of the THO complex; THO is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance (2, 3, 4, 5 and see Summary Paragraph)
Name Description Mitochondrial Fusion Targeting 1
Chromosomal Location
ChrXIII:148683 to 147505 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All MFT1 GO evidence and references
  View Computational GO annotations for MFT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
280 total interaction(s) for 183 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 73
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 13
  • Biochemical Activity: 6
  • Co-crystal Structure: 1
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 93
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 17
  • Synthetic Growth Defect: 35
  • Synthetic Lethality: 17
  • Synthetic Rescue: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 392
Molecular Weight (Da) 44,996
Isoelectric Point (pI) 4.26
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:148683 to 147505 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1179 148683..147505 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004527
SUMMARY PARAGRAPH for MFT1

MFT1 encodes a subunit of the THO complex, a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation by RNA polymerase II (2). Null mutations of any of the four THO complex genes are viable and exhibit similar phenotypes: a defect in transcription elongation, an increase in mitotic recombination between direct repeats (2), and defects in mRNA export (4, 3). The THO complex, along with Sub2p, Yra1p, and Tex1p, forms the transcription export (TREX) complex, conserved from yeast to metazoans and involved in the coupling of transcription to the export of the resulting mRNA (3).

There has been confusion surrounding the identity of the MFT1 gene and the function of its product. MFT1 was first thought to correspond to the RPS1B (YML063W) gene (1) but was later found to be the adjacent gene, YML062C (6). Mft1p was originally thought to be involved in mitochondrial import because an mft1 mutation was initially isolated in a genetic screen to identify mutations blocking the import of a deleterious Atp2p-lacZ fusion protein into mitochondria (1). Supporting this idea, Mft1p exhibits short regions of sequence similarity to Tom20p and Tom22p, which are mitochondrial import receptors, and also binds in vitro to peptides containing mitochondrial targeting sequences (7). However, the isolation of mft1 in the initial genetic screen appears to result from the fact that mutations in genes encoding subunits of the THO complex block transcription of lacZ (2), and the in vitro binding of Mft1p to mitochondrial targeting sequences appears not to be biologically relevant.

Last updated: 2003-02-25 Contact SGD

References cited on this page View Complete Literature Guide for MFT1
1) Garrett JM, et al.  (1991) Mitochondrial protein import: isolation and characterization of the Saccharomyces cerevisiae MFT1 gene. Mol Gen Genet 225(3):483-91
2) Chavez S, et al.  (2000) A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae. EMBO J 19(21):5824-34
3) Strasser K, et al.  (2002) TREX is a conserved complex coupling transcription with messenger RNA export. Nature 417(6886):304-8
4) Jimeno S, et al.  (2002) The yeast THO complex and mRNA export factors link RNA metabolism with transcription and genome instability. EMBO J 21(13):3526-35
5) Askree SH, et al.  (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
6) Beilharz T, et al.  (1997) The protein encoded by the MFT1 gene is a targeting factor for mitochondrial precursor proteins, and not a core ribosomal protein. FEBS Lett 407(2):220-4
7) Cartwright P, et al.  (1997) Mft52, an acid-bristle protein in the cytosol that delivers precursor proteins to yeast mitochondria. J Biol Chem 272(8):5320-5