PIF1/YML061C Summary Help

Standard Name PIF1 1, 2
Systematic Name YML061C
Alias TST1
Feature Type ORF, Verified
Description DNA helicase, potent G-quadruplex DNA binder and unwinder; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells (1, 3, 4, 5, 6, 7, 8)
Name Description Petite Integration Frequency 2
Chromosomal Location
ChrXIII:151532 to 148953 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PIF1 GO evidence and references
  View Computational GO annotations for PIF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant phenotypes All PIF1 Phenotype evidence and references
Classical genetics
null
overexpression
reduction of function
Large-scale survey
null
overexpression
Resources
interactions All PIF1 Interaction evidence and references
165 total interaction(s) for 103 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Reconstituted Complex: 2
Genetic Interactions
  • Dosage Growth Defect: 40
  • Dosage Lethality: 5
  • Dosage Rescue: 3
  • Phenotypic Enhancement: 39
  • Phenotypic Suppression: 22
  • Positive Genetic: 1
  • Synthetic Growth Defect: 16
  • Synthetic Lethality: 5
  • Synthetic Rescue: 22
Resources
Expression Summary
histogram
Resources
Protein Information All PIF1 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:151532 to 148953 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2580 151532..148953 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004526
References cited on this page View Complete Literature Guide for PIF1
1) Foury F and Lahaye A  (1987) Cloning and sequencing of the PIF gene involved in repair and recombination of yeast mitochondrial DNA. EMBO J 6(5):1441-9
2) Foury F and Kolodynski J  (1983) pif mutation blocks recombination between mitochondrial rho+ and rho- genomes having tandemly arrayed repeat units in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 80(17):5345-9
3) Ivessa AS, et al.  (2000) The Saccharomyces Pif1p DNA helicase and the highly related Rrm3p have opposite effects on replication fork progression in ribosomal DNA. Cell 100(4):479-89
4) Zhou J, et al.  (2000) Pif1p helicase, a catalytic inhibitor of telomerase in yeast. Science 289(5480):771-4
5) Vega LR, et al.  (2007) Sensitivity of yeast strains with long g-tails to levels of telomere-bound telomerase. PLoS Genet 3(6):e105
6) Guirola M, et al.  (2010) Lack of DNA helicase Pif1 disrupts zinc and iron homoeostasis in yeast. Biochem J 432(3):595-605
7) Crider DG, et al.  (2012) Rad53 is essential for a mitochondrial DNA inheritance checkpoint regulating G1 to S progression. J Cell Biol 198(5):793-8
8) Paeschke K, et al.  (2013) Pif1 family helicases suppress genome instability at G-quadruplex motifs. Nature ()