YME2/YMR302C Summary Help

Standard Name YME2 1
Systematic Name YMR302C
Alias RNA12 2 , PRP12 3
Feature Type ORF, Verified
Description Integral inner mitochondrial membrane protein; role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases (1, 4, 5, 6 and see Summary Paragraph)
Name Description Yeast Mitochondrial Escape 1
Chromosomal Location
ChrXIII:872625 to 870073 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All YME2 GO evidence and references
  View Computational GO annotations for YME2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Large-scale survey
95 total interaction(s) for 66 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Biochemical Activity: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Lethality: 2
  • Negative Genetic: 63
  • Phenotypic Enhancement: 1
  • Positive Genetic: 13
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 2
  • Synthetic Rescue: 3

Expression Summary
Length (a.a.) 850
Molecular Weight (Da) 96,688
Isoelectric Point (pI) 9.69
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:872625 to 870073 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2553 872625..870073 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004917

Yme2p is an integral mitochondrial inner-membrane protein (5). Conserved sequence elements in Yme2p suggest that it may function as an exonuclease (4). Some work suggests that Yme2p may play a role in maintaining the mitochondrial genome as well as a central role in controlling the mtDNA escape of some other yme mutants (4).

yme2 nulls exhibit a high rate of mtDNA escape and, depending on strain background, may also experience a respiratory defect due to the loss of mtDNA (5). Mutations in yme2 interact genetically with yme1 resulting in a synthetic respiratory growth defect on ethanol/glycerol media at 30 C (4). However, the gene products of YME1 and YME2 show no physical interactions with each other as assessed by two-hybrid analysis and co-immunoprecipitations (5).

Last updated: 2006-02-27 Contact SGD

References cited on this page View Complete Literature Guide for YME2
1) Thorsness PE and Fox TD  (1993) Nuclear mutations in Saccharomyces cerevisiae that affect the escape of DNA from mitochondria to the nucleus. Genetics 134(1):21-8
2) Liang S, et al.  (1992) rna12+, a gene of Saccharomyces cerevisiae involved in pre-rRNA maturation. Characterization of a temperature-sensitive mutant, cloning and sequencing of the gene. Mol Gen Genet 232(2):304-12
3) Liang, S. and Lacroute, F.  (1992) Personal Communication, Mortimer Map Edition 11
4) Hanekamp T and Thorsness PE  (1999) YNT20, a bypass suppressor of yme1 yme2, encodes a putative 3'-5' exonuclease localized in mitochondria of Saccharomyces cerevisiae. Curr Genet 34(6):438-48
5) Hanekamp T and Thorsness PE  (1996) Inactivation of YME2/RNA12, which encodes an integral inner mitochondrial membrane protein, causes increased escape of DNA from mitochondria to the nucleus in Saccharomyces cerevisiae. Mol Cell Biol 16(6):2764-71
6) Park S, et al.  (2006) Yme2p is a mediator of nucleoid structure and number in mitochondria of the yeast Saccharomyces cerevisiae. Curr Genet 50(3):173-82