SEN1/YLR430W Summary Help

SEN1 BASIC INFORMATION

Standard Name SEN1 1
Systematic Name YLR430W
Alias CIK3 , NRD2
Feature Type ORF, Verified
Description Presumed helicase required for RNA polymerase II transcription termination and processing of RNAs; homolog of Senataxin which causes Ataxia-Oculomotor Apraxia 2 and a dominant form of amyotrophic lateral sclerosis (2, 3, 4, 5 and see Summary Paragraph)
Name Description Splicing ENdonuclease 1
GO Annotations All SEN1 GO evidence and references
    View Computational GO annotations for SEN1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All SEN1 Phenotype details and references
Classical genetics
conditional
Large-scale survey
null
Interactions SEN1 All interactions details and references
65 total interaction(s) for 40 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 25
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 4
  • Biochemical Activity: 3
  • Two-hybrid: 18
Genetic Interactions
  • Dosage Rescue: 2
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 3
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 1
Sequence Information
ChrXII:993431 to 1000126 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2006-01-09 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..6696 993431..1000126 2006-01-09 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000004422

SEN1 RESOURCES

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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for SEN1

SUMMARY PARAGRAPH for SEN1

SEN1 encodes a Pol II transcriptional termination factor that is also involved in the maturation of noncoding RNAs (5, 2). Based on sequence similarity to and functional characterization of the S. pombe ortholog (also called sen1), S. cerevisiae Sen1p is presumed to be a 5'-3' ATP-dependent RNA/DNA helicase, although this activity has not been directly shown in S. cerevisiae (6, 7 and references therein). Sen1p localizes to the nucleus in a granular pattern (8). SEN1 is an essential gene but loss of function mutations in Sen1p cause altered accumulation of tRNA and ribosomal RNA precursors, mislocalization of core snoRNPs, and 3'-extension and transcriptional read-through of some snoRNAs, snRNAs, and short protein-encoding mRNAs (8, 7 and references therein). Mutations in the human homolog Senataxin (SETX), result in the progressive neurological diseases Ataxia-Oculomotor Apraxia 2 (AOA2) and juvenile Amyotrophic Lateral Sclerosis 4 (ALS4) (4 and references therein).

Last updated: 2007-09-07

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SEN1]

1) Winey M and Culbertson MR  (1988) Mutations affecting the tRNA-splicing endonuclease activity of Saccharomyces cerevisiae. Genetics 118(4):609-17
2) Ursic D, et al.  (1997) The yeast SEN1 gene is required for the processing of diverse RNA classes. Nucleic Acids Res 25(23):4778-85
3) Rasmussen TP and Culbertson MR  (1998) The putative nucleic acid helicase Sen1p is required for formation and stability of termini and for maximal rates of synthesis and levels of accumulation of small nucleolar RNAs in Saccharomyces cerevisiae. Mol Cell Biol 18(12):6885-96
4) Chen YZ, et al.  (2006) Senataxin, the yeast Sen1p orthologue: characterization of a unique protein in which recessive mutations cause ataxia and dominant mutations cause motor neuron disease. Neurobiol Dis 23(1):97-108
5) Steinmetz EJ, et al.  (2006) Genome-wide distribution of yeast RNA polymerase II and its control by Sen1 helicase. Mol Cell 24(5):735-46
6) Kim HD, et al.  (1999) The sen1(+) gene of Schizosaccharomyces pombe, a homologue of budding yeast SEN1, encodes an RNA and DNA helicase. Biochemistry 38(44):14697-710
7) Ursic D, et al.  (2004) Multiple protein/protein and protein/RNA interactions suggest roles for yeast DNA/RNA helicase Sen1p in transcription, transcription-coupled DNA repair and RNA processing. Nucleic Acids Res 32(8):2441-52
8) Ursic D, et al.  (1995) Inactivation of the yeast Sen1 protein affects the localization of nucleolar proteins. Mol Gen Genet 249(6):571-84