Standard Name	SPP382 1, 2
Systematic Name	YLR424W
Alias	CCF8 3 , NTR1 4
Feature Type	ORF, Verified
Description	Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation (2, 3, 4)
Name Description	Suppressor of PrP38 #2 2

Chromosomal Location	ChrXII:973395 to 975521 | ORF Map | GBrowse

	View Computational GO annotations for SPP382
Molecular Function
Manually curated	contributes_to ATP-dependent RNA helicase activity (IDA)
High-throughput	RNA binding (ISS)
Biological Process
Manually curated	generation of catalytic spliceosome for first transesterification step (IMP) spliceosomal complex disassembly (IDA, IGI, IMP)
High-throughput	mRNA splicing, via spliceosome (IPI)
Cellular Component
Manually curated	spliceosomal complex (IDA) U2-type post-mRNA release spliceosomal complex (IDA, IMP)
High-throughput	cytoplasm (IDA) mitochondrion (IDA) nucleus (IDA)

Classical genetics
conditional	colony sectoring: increased
null	inviable
reduction of function	unspliced RPS17A pre-mRNA accumulation: abnormal
Large-scale survey
conditional	heat sensitivity: increased
null	inviable
overexpression	vegetative growth: decreased rate
reduction of function	competitive fitness: increased resistance to benomyl: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	171 total interaction(s) for 86 unique genes/features.
Physical Interactions	Affinity Capture-MS: 79 Affinity Capture-Western: 13 Biochemical Activity: 1 Co-fractionation: 2 PCA: 3 Reconstituted Complex: 1 Two-hybrid: 36
Genetic Interactions	Dosage Lethality: 9 Dosage Rescue: 3 Negative Genetic: 17 Synthetic Growth Defect: 1 Synthetic Lethality: 4 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Mass Spec | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXII:973395 to 975521 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2127 973395..975521 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000004416