VRP1/YLR337C Summary Help

Standard Name VRP1 1
Systematic Name YLR337C
Alias END5 , MDP2 , YLR337W
Feature Type ORF, Verified
Description Proline-rich actin-associated protein; involved in cytoskeletal organization and cytokinesis; promotes actin nucleation and endocytosis; related to mammalian Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP) (1, 2, 3, 4)
Name Description VeRProlin 1
Gene Product Alias verprolin 5
Chromosomal Location
ChrXII:805106 to 802653 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All VRP1 GO evidence and references
  View Computational GO annotations for VRP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
conditional
null
Large-scale survey
null
overexpression
Resources
376 total interaction(s) for 272 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 6
  • Co-localization: 2
  • Far Western: 1
  • PCA: 15
  • Protein-RNA: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 31

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 9
  • Negative Genetic: 133
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 3
  • Positive Genetic: 92
  • Synthetic Growth Defect: 20
  • Synthetic Lethality: 33
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 817
Molecular Weight (Da) 82,593
Isoelectric Point (pI) 10.68
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:805106 to 802653 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2454 805106..802653 2004-02-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004329
References cited on this page View Complete Literature Guide for VRP1
1) Donnelly SF, et al.  (1993) A proline-rich protein, verprolin, involved in cytoskeletal organization and cellular growth in the yeast Saccharomyces cerevisiae. Mol Microbiol 10(3):585-96
2) Munn AL, et al.  (1995) end5, end6, and end7: mutations that cause actin delocalization and block the internalization step of endocytosis in Saccharomyces cerevisiae. Mol Biol Cell 6(12):1721-42
3) Thanabalu T and Munn AL  (2001) Functions of Vrp1p in cytokinesis and actin patches are distinct and neither requires a WH2/V domain. EMBO J 20(24):6979-89
4) Sun Y, et al.  (2006) Endocytic internalization in budding yeast requires coordinated actin nucleation and myosin motor activity. Dev Cell 11(1):33-46
5) Roumanie O, et al.  (2000) Evidence for the genetic interaction between the actin-binding protein Vrp1 and the RhoGAP Rgd1 mediated through Rho3p and Rho4p in Saccharomyces cerevisiae. Mol Microbiol 36(6):1403-14